Special Issues-07-01-2016

Water samples were obtained from the Tar River and a local water treatment plant in eastern North Carolina in spring 2013 and fall 2015 to monitor the presence of a panel of pharmaceutical and personal care products (PPCPs). Samples were extracted by solid phase extraction (SPE) or liquid-liquid extraction and analyzed for parent PPCPs and their metabolites by liquid chromatography-time of flight mass spectrometry (HPLC-TOFMS) and gas chromatography-mass spectrometry (GC-MS). Both extraction and detection methods were compared by their recoveries and detection limits for each compound. Many parent PPCPs and their metabolites were detected including: carbamazepine, iminostillbene, oxcarbazepine, epiandrosterone, loratadine, β-estradiol, triclosan, and others. Liquid-liquid extraction was found to give overall superior recoveries. Furthermore, HPLC-TOFMS gave lower detection limits than GC-MS. Library searching of additional peaks identified further compounds with biological activity. Additionally, the effectiveness of the treatment plant on the removal of the compounds of interest is discussed.

An LC-MS method has been developed for simultaneous quantification of buprenorphine and its three metabolites, namely norbuprenorphine, buprenorphine glucuronide, and norbuprenorphine glucuronide Chromatographic separation was achieved on a C18 column with a gradient of acetonitrile and ammonium acetate buffer (25 mM, pH 6.6). The method run time was 7.5 min. Quantification was performed by selected ion monitoring of [M+H]+ ions of norbuprenorphine glucuronide (590), norbuprenorphine (414), buprenorphine glucuronide (644) and buprenorphine (468). Naloxone (328) (328ng/mL) was used as an internal standard. The samples were processed by protein precipitation and extraction recovery was ≥95% with minimal observed matrix effects (

In this study we report on the use of a field-portable GC-MS with rapid sampling techniques such as solid-phase micro extraction, purge-and-trap, thermal desorption, and heated headspace to provide a fast response for in-field-SVOCs analyses for a wide variety of environmental-type samples including potable waters, tea, plants and road gravel. We will show that this field-portable approach can provide the required sensitivity, selectivity for the effective analysis of SVOCs with very high boiling points such as polycyclic aromatic hydrocarbon (PAHs), pesticides, phenolic compounds and phthalate esters in a number of different field-based samples, in less than 10 minutes.

Moxidectin formulations help to reduce hair loss and irritation due to parasite worms in animals. So Estimation of Moxidectin in hair is important to evaluate therapeutic levels, distribution & accumulation, however estimation is also useful to evaluate harm to birds when they eat animal hair. Hence Moxidectin estimation is required for pharmacokinetic as well as environmental exposure study. Objective of the present work is to develop a rapid, selective method for the estimation of Moxidectin in Cattle Hair by LC-MS/MS. Oxcarbazepine used as a internal standard. Moxidectin extracted from cattle hair by liquid-liquid extraction using Sorenson’s Buffer as digestion solvent for incubation & methyl tert-butyl ether as an extraction solvent. Detection was performed over the range 0.026 to 1.000 ng/mG using MRM in positive polarity at unit resolution under turbo ion spray whereas separation was achieved on Kinetex 100 x 4.6 mm, 5u EVO C18 100A column with Methanol : 10mM Amonium formate pumped as gradient flow with 4.50min run time. Q1 is 640.45 whereas Q3 is sum of 528.50 and 498.50. Validation parameters shown reliable results. Method is applied for the estimation of Moxidectin in cattle Hair.

We present a brief review of this year's ASMS conference, which took place June 5–9 in San Antonio, Texas.

The Diablo 5000A RTGA-MS allows the process stream to be seen in real-time and provides quantitative data with reliable mass information. The result is visualization.

This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.

Click the title above to open the July 2016 issue of Current Trends in Mass Spectrometry, Volume 14, Number 3, in an interactive PDF format.