ParticleScout for Automated Raman Imaging Analysis of Microparticles (Updated)

The Cary 3500 UV-Vis systems use temperature-controlled UV-Vis spectroscopy to analyze molecules' thermal stability. They are essential for nucleic acid quantification quality control by measuring absorbance at 260 nm and performing thermal melt identification verification. Melting temperature (Tm) analysis is key for studying nucleic acid denaturation.

All starting materials, including APIs, must undergo analytical identity (ID) determination before pharmaceutical manufacturing to ensure drug safety. Oligonucleotides pose challenges for ID due to sequence specificity. Melting temperatures (Tm) combined with intact mass measurements offer a solution using UV-based spectrometry for precise Tm determination at Vetter Pharma.

Vetter Pharma successfully uses the Cary 3500 UV-Vis to optimize thermal melt measurements of oligonucleotide-based pharmaceuticals.

Characterizing biomolecules such as oligonucleotides and proteins is critical to better understanding biochemical and molecular processes. UV-Vis spectroscopy is widely used for stability studies, protein aggregation, DNA quantification and purity, and enzyme activity. The Cary 3500 UV-Vis offers advanced features to provide accurate and reproducible results.

The drug discovery process involves identifying and characterizing drug targets to find lead compounds. Biochemical characterization (e.g., molecular weight, solubility, thermal stability) is crucial for protein therapeutics. Thermal stability studies measuring melting temperatures help understand protein stability, ligand-binding, and optimal storage, aiding drug development and commercialization.