The Agilent Cary 60 UV-Vis spectrophotometer is the new, improved successor to the award-winning Cary 50 UV-Vis. In this short review, this instrument platform was evaluated for its potential to measure small (40 µL) samples of methylene blue in situ during exposure to high-intensity UV irradiation. Methylene blue is combined with other compounds used in a variety of applications, including use in cosmetics and sun screen products and environmental remediation in contaminated air and polluted water.