LC-MS Used to Analyze Poppy Alkaloid Metabolism

Article

A team from the University of Calgary and the National Research Council Plant Biotechnology Institute has been studying the alkaloid metabolism process that commonly takes place in opium poppy cell cultures, using liquid chromatography and mass spectrometry (LC-MS)

A team from the University of Calgary and the National Research Council Plant Biotechnology Institute has been studying the alkaloid metabolism process that commonly takes place in opium poppy cell cultures, using liquid chromatography and mass spectrometry (LC-MS).

The team published their findings in BMC Plant Biology, an open-access publication, where they explained how using LC-MS enabled them to analyze protein extracts within cell cultures that had been treated using a fungal elicitor.

The poppy flower, from which several different pharmacological solutions like codeine and morphine are commonly derived from, contains 1,004 different proteins, identified and analyzed using LC-MS.

The scientists were able to compile an opium poppy-specific expressed sequence tag database, allowing them to greatly improve their identification of the different proteins.

Recent Videos
Lake Tahoe West shore view including Fannette Island in the winter of 2018 | Image Credit: © AlessandraRC - stock.adobe.com.
North Lake Tahoe Sunset | Image Credit: © adonis_abril - stock.adobe.com.
North Lake Tahoe Sunset | Image Credit: © adonis_abril - stock.adobe.com
Beautiful Day in Lake Tahoe, California | Image Credit: Jeremy Janus - stock.adobe.com
Beautiful Day in Lake Tahoe, California | Image Credit: © Jeremy Janus - stock.adobe.com
Sand Harbor Lake Tahoe Nevada | Image Credit: © Stephen - stock.adobe.com.
Abstract molecular structure with green background and sun glare. Generated by AI. | Image Credit: © Edi Purnomo - stock.adobe.com.
The country Sierra Leone is in West Africa, and benefits from a tropical Atlantic coastline. Its bordered by Guinea and Liberia. The Capital is Freetown, and you can head to the beach in Sierra Leone. | Image Credit: © KALADA - stock.adobe.com.
Modern video camera recording tv studio interview blurred background mass media technology concept | Image Credit: © Studios - stock.adobe.com.