Christopher A. Evans

The collection of blood-derived samples from preclinical and clinical trial studies onto paper-based, Guthrie-type cards is gaining momentum within the pharmaceutical industry. This approach holds the potential to minimize animal usage, improve data quality, and reduce shipping costs. However, the small sample volumes and extra matrix effects from the cards result in method development and sensitivity challenges for bioanalysts. Here, we present a discussion on the analytical challenges that both liquid chromatography and mass spectrometry face as well as present some potential solutions to these issues.