Detection and Characterization of Extractables in Food Packaging Materials by GC–MS
July 1st 2017In this study, general extract screening of food storage materials was done with nontargeted analytical methods to understand what analytes could potentially leach into food or beverages. GC and mass spectral deconvolution effectively separated analytes within the complex mixture and TOF-MS provided full mass range spectral data for identification. This workflow can be used for confident characterization of components present as extractables from food packaging materials.
Determination of Very Low Abundance Diagnostic Proteins in Serum Using Immunocapture LC–MS/MS
July 1st 2017There is growing interest in the determination of endogenous proteins in biological samples for diagnostic purposes, because a concentration increase or decrease of such proteins can allows us to monitor the state of a pathological condition such as cancer. Immunocapture LC–MS/MS analysis combines the workflow of conventional immunological assays with LC–MS analysis. This article describes typical challenges, such as cross reactivity and the mass spectrometer’s dynamic range, as well as the advantages of isoform differentiation and multiplexing.
Ion Mobility Spectrometers as Chromatographic Detectors
July 1st 2017Interest in connecting ion mobility spectrometry (IMS) to GC and especially to LC is now growing. One favorable property of IMS is that it can work with ambient pressure and can be easily connected to a gas or liquid chromatograph. Analytical applications of GC–MS and LC–MS are very different and encompass investigations into food, medical science, environment, drugs of abuse, chemical warfare agents, and explosives.
Quantitative Drug Metabolite Profiling without Radiolabels Using HPLC–ICP-MS
July 1st 2017In drug development, quantitative determination of a candidate drug and its metabolites in biofluids is an important step. The standard technique for quantitative metabolite profiling is radiolabeling followed by HPLC with radiodetection, but there are disadvantages to this approach, including cost and time, as well as safety and ethical concerns related to administering radiolabeled compounds to humans. Frank Vanhaecke and his research group at Ghent University have been developing an alternative technique, and he recently spoke to us about this work.
Rapid Perfluorinated Alkyl Acid Analysis by LC–MS/MS Increases Sample Throughput
July 1st 2017• Raptor C18 SPP 5 μm core-shell silica particle columns offer excellent resolution for fluorochemicals with short total cycle times. For even faster analysis, 2.7 μm core-shell particles are available. • Meets EPA Method 537 requirements. • Unique, robust Raptor C18 column design increases instrument uptime.