Improved UV-Vis Applications: From Simultaneous Measurements to Fast Thermal Melts

Join us to learn about better workflows for UV-vis analysis, to improve efficiency in data collection and sample throughput with simultaneous data collection, and how to develop sample analysis methods for determination of material properties like concentration and melting temperature.

Register free: https://www.spectroscopyonline.com/spec/melts

Event Overview:

UV-Vis spectrometers are very flexible instruments, with the ability to perform several different qualitative and quantitative sample measurements. Besides performing basic scans, applications can include creating calibration curves of absorbance versus concentration to identify unknown samples, measuring absorbance over time to study reactions, and studying absorbance as a function of temperature to perform melt experiments.
In this webinar, improved workflows for UV-Vis spectroscopy will be discussed. Discover how to develop Beer-Lambert Law calibration curves for determining the concentrations of unknown BSA samples as well as how to how to monitor thermal melts and chemical reactions using UV-Vis spectroscopy. For the examples discussed in this presentation, an Agilent Cary 3500 UV-Vis spectrophotometer will be used. The benefits of data collection across eight cuvette positions and measuring up to four temperature experiments, all simultaneously, will be explored. The samples that will be discussed here were run using an integrated air-cooled Peltier system allowing for fast data ramping possibilities as well as a xenon flash lamp achieving a data collection rate of 250 points per second.


Key Learning Objectives:

• Learn to perform routine UV-Vis analyses
• Build a better workflow for improving efficiency in data collection and sample throughput with simultaneous data collection
• Develop sample analysis methods for determination of material properties like concentration and melting temperature

Who Should Attend:

• UV-Vis spectroscopists
• Analytical chemists
• Pharmaceutical scientists

Speaker

Scott Melis, PhD
Molecular Spectroscopy Applications Scientist
Agilent Technologies

Scott Melis has a PhD in Physics from Georgetown University in Washington, DC, and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications.Projects that he worked on include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.

Register free: https://www.spectroscopyonline.com/spec/melts