Application Notes

As the temperature increases, significant changes in the myoglobin secondary structure are observed. Between 25 °C and 70 °C, the 1649 cm-1 peak height decreases and linewidth increases. These changes in the amide I band reflect increased protein structural disorder. A temperature increase from 70 °C to 75 °C causes a drop in the 1649 cm-1 band intensity. Simultaneously, two peaks grow in at 1683 and 1637 cm-1. These changes indicate conversion of random coil/α-helix to intermolecular β-sheet. Further conversion to β-sheet is observed as the temperature rises to 90 °C. The final β-sheet structure is an insoluble aggregate. This aggregation process is not reversed when the temperature is driven back to 25 °C.

Single reflection ATR spectroscopy is valuable for examining thermally induced changes in materials. Changes in the water concentration and amide bands of egg yolk were observed using the Harrick DiaMaxATR accessory. Further analysis is possible to obtain more rigorous identification of the heat-induced structural changes.

The flavonoids that dominate the fluorescence spectra for honey shown in Figure 1 are polyphenols. These plant metabolites determine the color, aroma, and flavor of the honey, and provide antioxidant and other health benefits. The fluorescence spectrum for each honey sample illustrates the sensitivity of fluorescence spectroscopy for characterizing honey.

The LUMOS FT-IR microscope is an all-in-one solution with an integrated spectrometer, a high degree of automation, and a dedicated user interface. The innovative motorized attenuated total reflectance (ATR) crystal allows performing the complete measurement procedure fully automated including background and sample measurements. The dedicated OPUS software Wizard guides the user through the whole measurement procedure. For identification of unknown compounds, spectrum search and mixture analysis are applied using the functionality of the OPUS/SEARCH package. Chemical images of the spectral data are generated from univariate and multivariate analysis results.

RISE microscopy, the combination of Raman imaging and scanning electron microscopy, is a powerful new analytical method for the analysis and interrelation of the structure and composition of microscopic samples. The integration of both imaging techniques in one instrument avoids shuttling the sample from one microscope to another. Here we demonstrated its usefulness in the identification, discrimination, and localization of polymorphs of titanium dioxide.

Download this application note to learn how to rapidly and accurately determine arsenic in apple juice using HPLC/ICP-MS.

Modern paints and varnishes are complex mixtures and the optimization of the material properties is an important task. One of the most essential variables is the curing which should in most cases take place in a reasonably short time span that can range from a fraction of a second to many days. It is difficult to evaluate the degree of the curing and its completeness by visual inspection. Therefore, an unbiased and reliable quantitative analysis is needed in quality control but also in R&D to optimize the varnish or the curing method respectively.

In this application note, we focus on identification of adulterants in ground spices. Reflectance spectra collected using Ocean Optics spectroscopic systems can reveal features signaling common spice adulterants, providing rapid on-site quality assessment without the need for chemical analysis.

Learn how to achieve reliable accuracy, reduced test time, and reproducibility, when using thermogravimetry to quantify the components of automotive rubber products, namely oil, polymer, carbon and inorganic filler.

Raman spectroscopy provides data on bone mineral crystallinity, carbonate to phosphate ratio and extent of collagen cross-linking, all compositional information that is intimately related to biomechanical characteristics of this unique material. Key words for lead email subject line: Raman spectroscopy on bones

Indocyanine Green fluorescent dye displays fluorescence in the near infrared region in the vicinity of 810 nm; however, measurement in wavelength regions greater than 750 nm has been difficult. This note presents an example of measuring Indocyanine Green with good sensitivity while obtaining corrected spectra in real time.

For all measurements, a Harrick Wire Grid Polarizer (KRS-5 substrate) was used. The polarizer was oriented in the same position for both background and sample measurements. All spectra were collected using an FT-IR spectrometer at an 8 cm-1 resolution averaged over 32 scans.

In this application note, we focus on the peripheral artery disease (PAD) and its treatment, utilizing NeoFox optical oxygen sensor system to demonstrate how oxygenation increases stem cell survival and leads to improved therapeutic outcomes for PAD patients.

An infrared transmission spectrum of lens tissue was collected and compared with a sample spectrum collected in the FT-IR sample compartment. Benefits of using an integrating sphere are discussed.

Combined investigations in the far and mid infrared region in a single scan can offer great value for the analysis of polymeric materials. With the use of optical components optimized for combined FIR-MIR spectroscopy, consisting of a wide range mid to far IR beamsplitter and detector, the investigation of both spectral regions in single step is possible.

Learn how to measure properties of Gold Nanoparticles that have unique optical, electronic, and thermal properties and used in a wide range of applications including diagnostics assays, microscopy and electronics.

BaySpec’s OCI-UAV hyperspectral imagers are ultra-compact, easily deployable, high-performance hyperspectral cameras designed specifically for use on small unmanned aerial vehicles (UAVs), to monitor the details of spatial, temporal, and spectral representation of ground objects.

Data for a handheld LIBS device (SciAps Z-500) is presented demonstrating in-field measurement of several elements critical for geochemical applications. In some cases these elements have never before been measured with a handheld device.

Learn how the spectral characterization of Enhanced Spectral Reflector (ESR) films is an important step in developing new novel applications for these unique materials. From enhanced skylights to brighter displays ESR films play an important role.

In addition to fluorescence avoidance, 1064nm Raman reduces stringent sampling conditions necessary at shorter wavelengths. Concerns about low Raman scattering cross-sections can be totally compensated by increased laser power.

CEM's Discover SP-D Gold microwave digestion system with high temperature and pressure chamber enables safe, fast, and automated preparation of previously difficult sample types

The 12 W miniature source has a higher voltage and a much higher emission current than our present miniature X-ray sources, which is an advantage for a number of applications.

The majority of environmental laboratories are currently using traditional techniques like cold vapor atomic absorption to analyze samples for mercury content.

The TSI® LIBS Desktop Analyzer laser induced breakdown spectroscopy (LIBS) instrument allows simultaneous determination of all detectable elements present in the material being analyzed.

Portable 785 nm and 1064 nm excitation wavelength dispersive Raman instruments have proven their worth in materials identification, especially for forensics, pharmaceutical raw materials inspection, and first responders.

Inks and pigments with optically unique properties can provide quick visual verification of authenticity, and can be difficult to replicate.

In the agri-food sector it is important to not only study the soil composition and use of fertilizers, but also the uptake of nutrients and potentially toxic elements within the plants and crops themselves.

Elemental ratio determinations in foraminiferal calcite require the measurement of trace levels of Na, Mg, Sr, and ultra-trace levels of Li, B, Ba, Cd, Mn, U in a calcium rich matrix and ratioed to calcium.

Monolayers and other thin coatings on Si and other high refractive index substrates are of key importance in materials science, bioscience, synthetic organic, and semiconductor research.

Measuring the transmission of diffuse samples such as paper or filled polymer films requires an integrating sphere to obtain accurate transmission values and useful chemical information, because the IR light is scattered as it passes through the sample. An integrating sphere serves as a collection device for the scattered light.