Application Notes: General

Learn about the different materials that can be used as internal reflection elements for ATR spectroscopy.

Learn about the different window materials available for use in Specac products, including the Omni-Cell, Pearl, High-Temperature High-Pressure (HTHP) and Heatable Liquid Cells.

BLADE was used to digest various food samples, including those with high fat content, high protein content, and even high carbohydrates, followed by analysis using ICP-MS.

A polymer film was measured at Brewster’s angle to eliminate interference fringing in the IR spectrum. This technique is beneficial for the analysis of additives.

The advantages of single reaction chamber (SRC) microwave digestion are presented and exemplified by its application to sample preparation for food, advanced materials, mining and geochemistry, and consumer goods analyses.

This whitepaper describes applications of cryoRaman, a cryogenic Raman microscope offering VIS to NIR excitation, high magnetic fields, and full polarization control.

Ensuring consistent quality of dairy solids during processing has importance in consumer goods and nutritional supplements.

Learn how this efficient and effective analytical technique aids in monitoring chemical reactions and maintaining optimal conditions throughout bioprocessing and chemical manufacturing.

Direct determination of 21 elements in mixes of LIB-solvents DMC, EMC, and EC

The elemental content of single yeast cells and polystyrene microbeads was analyzed using an Agilent 8900 ICP-QQQ with ESI microFAST SC sample introduction system.

Analysis of cathode raw materials for Li-ion batteries by Agilent 5900 ICP-OES

Learn practical aspects of analyzing microplastics with the Agilent 8700 LDIR and dealing with the difficulties of analyzing particles smaller than 20µm.

Learn how sub-micron resolution IR microscopy and high-speed spectroscopy overcomes the limitations of Raman spectroscopy for advanced failure analysis applications.

Learn how O-PTIR spectroscopy overcomes the limitations of Raman, providing automated measurement and chemID of sub-micron sized microplastics.

Learn how simultaneous O-PTIR and Raman provide high resolution IR imaging and spectroscopy of multilayer laminate film with minimal sample preparation.

Explains how O-PTIR spectroscopy overcomes the limitations of Raman spectroscopy by measuring defects in recessed semiconductor packaging structures.

Learn how sub-500nm resolution O-PTIR and co-located fluorescence microscopy combine to offer new insights and breakthroughs for life science research.

This application shows the depth profiling of mobile phone touch screens, facilitating visualization of the layered structure to illustrate technology advances.

Learn new ways to optimize molecular analysis with flexible, software-defined lock-in detection and stimulated Raman scattering (SRS) techniques. This powerful combination delivers unparalleled sensitivity, background-free imaging, and real-time insights so you can achieve more, faster.

The Geno/Grinder is a high-throughput plant and animal tissue homogenizer and cell lyser. It is equipped with an adjustable clamp that accommodates a full range of sample vials from 2 mL to 50 mL centrifuge tubes or up to six deep-well titer plates. It is specifically designed for rapid cell disruption, cell lysis and tissue homogenization through bead beating. This enables fast and efficient extraction of nucleic acids, proteins and other molecules. The Geno/Grinder incorporates a password-protected, touch screen control panel, enabling the user to program run time, rate, cycles and pause time.

Sample preparation is the process where a representative piece of material, chemical or substance is extracted from a larger amount, bulk or batch for subsequent analysis. Representative samples are selected to accurately reflect the larger group and represent the characteristics of the whole material. Ideally representative samples are homogeneous or similar in nature, but when that is not possible, the best attempts must be made to achieve samples which represent the majority of the characteristics of the larger grouping. The preparation of samples is one of the most important steps in analytical methods for many reasons, including the fact that some materials cannot be analyzed in an in-situ condition (such as proteins, DNA and RNA). Some samples have interfering substances and species that can produce faulty results. Sample preparation can include many processes, from reactions or treatment with chemical agents, to filtration, dilution, and extraction.

The Cryo-Blade is an ultra high-capacity cryogenic grinder for comminution and processing capability up to 1 kg. The homogeneity of the sample is very efficient. Only 1 g is needed as a representative test sample from a 1 kg ground batch. The high-throughput capability saves valuable time for your laboratory and meets Food and Drug Administration (FDA) regulations. The Cryo-Blade automatically dispenses liquid nitrogen to allow the sample to reach cryogenic temperatures and then activates a grinding phase to pulverize samples, resulting in repeatable and reproducible sample preparation. No liquid nitrogen handling required. When liquid nitrogen is not desired or available, the unit can be used with dry ice to achieve desired results.

Bead beating is an effective process used to disrupt a wide range of biological samples. It is achieved by rapidly agitating samples with grinding media (balls or beads) in a bead beater. Samples can be processed with or without buffer or solvent at room temperature or cryogenically. The Geno/Grinder® and MiniG® are high-throughput homogenizers that can process samples in deep well plates as well as other formats (up to 50 mL tubes). The homogenizers have a linear motion that focuses the kinetic energy of the grinding media on the sample instead of on the sides of the container. Consequently, even resilient samples such as seeds are most effectively homogenized by these linear motion bead beaters.

From entry-level items for the academia market to specialized instruments for the pharmaceutical, life sciences, chemical analysis, environmental testing, diagnostics and healthcare sectors, Cole-Parmer® offers high quality solutions for years of trouble-free operation.

Discover PerkinElmer's comprehensive and innovative solutions for the identification and quantification of microplastics.

This case study focuses on the University of Birmingham, where a UK-based research team is making great strides in microplastics analysis.