Authors



Victor Mizrahi

Latest:

Molecular Spectroscopy Workbench: Thin-Film Filters for Raman Spectroscopy (PDF)

Recent advances in thin-film filter technology have enabled dramatic improvements in the performance of filters for laser-based analytical instrumentation.


Tosoh Bioscience

Latest:

Analysis of a Bispecific Antibody Using SEC–MS

This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.


Millipore

Latest:

Atomic Spectroscopy in Practice: A Joint Virtual Conference Presented by Spectroscopy Magazine and The Society for Applied Spectroscopy

***Live: Tuesday February 23, 2021 & Wednesday February 24, 2021*** Spectroscopy magazine and the Society for Applied Spectroscopy (SAS) are pleased to present a joint virtual conference on February 23 and 24, 2021, called “Atomic Spectroscopy in Practice.” This two-day event will provide highly practical information for atomic spectroscopists and analytical laboratory staff working with molecular atomic techniques. *** On demand available after final airing until Feb. 24, 2022***



Poul Hansen

Latest:

How to Design a Miniature Raman Spectrometer

In this article, we describe the key factors that influence the overall size of a spectrometer, such as the diffraction grating groove density and detector size. Furthermore, we demonstrate compact Raman spectrometer designs as small as 30 mm × 30 mm in footprint by using highly dispersive gratings and uncooled detectors.




Tsukasa Shishika

Latest:

A New Tool for Mass Analysis of Unknown Molecules: High-Resolution Multistep Tandem MS with Wide Dynamic Range Quantitative Analysis

Mass spectrometers are effective for identifying and quantifying unknown molecules, such as disease-related proteins and small molecules in pharmaceutical research and medical diagnosis. In addition, mass spectrometry (MS) can be particularly powerful when analyzing molecules with complex structures, such as posttranslationally modified proteins. Among various MS approaches, high-resolution multistep tandem MS (MS-MS) is an emerging methodology for accurate identification of complex molecules. In this article, we describe a new approach for mass analysis with enhanced quantitative capability combined with high-resolution multistep MS-MS, where the dynamic range of quantitation covers four orders of magnitude.



Gary Harland

Latest:

Improving on GC–MS Performance for Demanding Applications: The Use of GC Coupled to Tandem-Quadrupole MS

Gas chromatography–mass spectrometry (GC–MS) and liquid chromatography (LC)–MS are widespread successful approaches, based on single-quadrupole MS, for the routine detection, identification, and quantitation of compounds. There has, however, been increasing interest in the use of tandem MS in more challenging, complex matrices such as those commonly found in food, environmental, and biological analyses. The combination of GC with tandem-quadrupole MS (MS-MS) is discussed, where the inherent increase in selectivity and sensitivity of the approach has enabled rapid, confident compound detection and quantitation for such demanding applications.


Scott Peterman

Latest:

Targeted Quantitative Protein Analysis in Human Serum Using High-Resolution Multiple Selected Reaction Monitoring Assays

A common endpoint for a biomarker discovery experiment is a list of putative marker proteins. The next step is then to perform targeted quantitative measurements of these proteins in an expanded patient population to assess their validity as markers. Analytical accuracy and precision are required for unambiguous quantitative analysis of targeted proteins from very complex mixtures. Wide dynamic range and high sensitivity are critical for detecting low-abundance proteins. Such an assay also is appropriate for "targeted discovery" experiments, where the goal is to quantitate a large number (up to hundreds) of known proteins in a complex sample.





Ken Miller

Latest:

Targeted Quantitative Protein Analysis in Human Serum Using High-Resolution Multiple Selected Reaction Monitoring Assays

A common endpoint for a biomarker discovery experiment is a list of putative marker proteins. The next step is then to perform targeted quantitative measurements of these proteins in an expanded patient population to assess their validity as markers. Analytical accuracy and precision are required for unambiguous quantitative analysis of targeted proteins from very complex mixtures. Wide dynamic range and high sensitivity are critical for detecting low-abundance proteins. Such an assay also is appropriate for "targeted discovery" experiments, where the goal is to quantitate a large number (up to hundreds) of known proteins in a complex sample.




He'an Luo

Latest:

Resonance Light Scattering Spectrum of the Alizarin Green-CTMAB-Nucleic Acids System and Determination of Nucleic Acids at Nanogram Levels

The study of the interaction mechanism of nucleic acids-CTMAB with AG showed that the enhanced RLS comes from the aggregation of AG on nucleic acids through the bridged and synergistic effect of CTMAB.


Yuichiro Hashimoto

Latest:

Electron-Capture Dissociation in a Radio-Frequency Linear Ion Trap

Here we describe a new compact device for electron-capture dissociation (ECD) analysis of large peptides and posttranslational modifications of proteins, which can be difficult to analyze via conventional dissociation techniques such as collision-induced dissociation (CID). The new compact device realizes ECD in a radio frequency (RF) linear ion trap equipped with a small permanent magnet, which is significantly different than the large and maintenance-intensive superconducting magnet required for conventional ECD in Fourier-transform ion cyclotron resonance mass spectrometers. In addition to its compactness and ease of operation, an additional merit of an RF linear ion trap ECD is that its reaction speed is fast, comparable to CID, enabling data acquisition on the liquid-chromatography (LC) time scale. We interfaced the linear-trap ECD device to a time-of-flight mass spectrometer to obtain ECD spectra of phosphorylated peptides injected into a liquid chromatograph, infused glycopeptides, and intact small..


Hideki Hasegawa

Latest:

Electron-Capture Dissociation in a Radio-Frequency Linear Ion Trap

Here we describe a new compact device for electron-capture dissociation (ECD) analysis of large peptides and posttranslational modifications of proteins, which can be difficult to analyze via conventional dissociation techniques such as collision-induced dissociation (CID). The new compact device realizes ECD in a radio frequency (RF) linear ion trap equipped with a small permanent magnet, which is significantly different than the large and maintenance-intensive superconducting magnet required for conventional ECD in Fourier-transform ion cyclotron resonance mass spectrometers. In addition to its compactness and ease of operation, an additional merit of an RF linear ion trap ECD is that its reaction speed is fast, comparable to CID, enabling data acquisition on the liquid-chromatography (LC) time scale. We interfaced the linear-trap ECD device to a time-of-flight mass spectrometer to obtain ECD spectra of phosphorylated peptides injected into a liquid chromatograph, infused glycopeptides, and intact small..


Elke Brouwers

Latest:

ICP-MS: When Sensitivity Does Matter

It makes intuitive sense - the higher the sensitivity of an inductively coupled plasma–mass spectrometry (ICP-MS) system, the lower the detection limit. But there are many factors that affect the detection limit for a given isotope in a given sample. These factors include sensitivity, background noise, and interferences.


Hiroyuki Satake

Latest:

Electron-Capture Dissociation in a Radio-Frequency Linear Ion Trap

Here we describe a new compact device for electron-capture dissociation (ECD) analysis of large peptides and posttranslational modifications of proteins, which can be difficult to analyze via conventional dissociation techniques such as collision-induced dissociation (CID). The new compact device realizes ECD in a radio frequency (RF) linear ion trap equipped with a small permanent magnet, which is significantly different than the large and maintenance-intensive superconducting magnet required for conventional ECD in Fourier-transform ion cyclotron resonance mass spectrometers. In addition to its compactness and ease of operation, an additional merit of an RF linear ion trap ECD is that its reaction speed is fast, comparable to CID, enabling data acquisition on the liquid-chromatography (LC) time scale. We interfaced the linear-trap ECD device to a time-of-flight mass spectrometer to obtain ECD spectra of phosphorylated peptides injected into a liquid chromatograph, infused glycopeptides, and intact small..


Michelle Nicolson

Latest:

PittCon 2002 Review (PDF)

Spectroscopy's annual overview of new instrumentation presented at the PittCon conference.


Jenni L. Briggs

Latest:

Non-Destructive FT-IR Measurements via Diffuse Reflection Sampling

For analysis of non-particulate solids, the diffuse reflection sampling technique may offer an easy, non-destructive method for mid-infrared measurements. Spectral results of a polypropylene face mask collected via diffuse reflection and attenuated total reflection (ATR) were compared.



PIKE Technologies, Inc

Latest:

Polymer processing influences on FTIR-ATR results

The polymer processing can impact FTIR-ATR spectra, and if these processing factors are not considered may lead to incomplete or inconsistent results.



Heinz P. Singer

Latest:

Online Automated SPE–LC–MS-MS Quantitation of Sulfonamide Antibiotics and Pesticides in Surface Waters Using a Triple-Quadrupole Mass Spectrometer and Electrospray Ionization Probe

This article describes a fully automated online solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE–LC–MS-MS) setup using a mass spectrometer and an electrospray ionization probe for analyzing different groups of polar contaminants in natural waters. The goal was to develop an online SPE method for the quantification of sulfonamide antibiotics, including their acetyl metabolites, as well as for frequently used pesticides (triketones, phenylureas, chloracetanilides, phenoxyacetic acids, amides, and triazines) in ambient waters. The analytical methods were applied successfully for a field study in an agricultural region within the catchment area of Lake Greifensee near Zurich, Switzerland.


Michael MacRae

Latest:

Trends in Spectroscopy: A Big-Picture Look at Recent Innovations and Future Directions

Recent federal science and technology initiatives are focusing public attention and funding on the need for innovative research in environmental quality, sustainability, medicine, and advanced materials.