Authors

Latest:

BaySpec’s PeakFinderTM Raman video probe combines a flexible fiber optic probe with microscope objectives allowing sampling down to several microns. Combined with various benchtop Raman spectrometers, the Raman video probe is a unique micro-Raman system available in 532, 785 or 1064nm excitation and various microscope objectives offering unprecedented flexibility and versatility.

Latest:

BaySpec’s PeakFinderTM Raman video probe combines a flexible fiber optic probe with microscope objectives allowing sampling down to several microns. Combined with various benchtop Raman spectrometers, the Raman video probe is a unique micro-Raman system available in 532, 785 or 1064nm excitation and various microscope objectives offering unprecedented flexibility and versatility.

Latest:

BaySpec’s PeakFinderTM Raman video probe combines a flexible fiber optic probe with microscope objectives allowing sampling down to several microns. Combined with various benchtop Raman spectrometers, the Raman video probe is a unique micro-Raman system available in 532, 785 or 1064nm excitation and various microscope objectives offering unprecedented flexibility and versatility.

Latest:

Despite all of the recent advances in analytical technologies dedicated to biotherapeutics, accurate protein quantification remains a challenge for the biopharmaceutical industry. UV spectrophotometry is commonly used for batch testing, but it requires the knowledge of the extinction coefficient of the protein, whose experimental determination requires the accurate concentration of a reference standard obtained by an absolute quantification method. To address the need for a fast analytical method capable of accurately quantifying a protein without any specific reference substance, an isotope dilution ICP-MS method was developed and validated, based on sulfur determination, allowing very accurate determination of a single protein in solution after microwave digestion.

Latest:

We have developed a range of analytical workflows using mass spectrometry, in a regulated environment, to support pharmaceutical companies in the development and control of their monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs). High-resolution mass spectrometry is a powerful tool for the analysis of antibodies, but is not readily compatible with a number of chromatographic techniques using high-salt mobile phases. Herein, we present the development and use for marketed mAbs and ADCs of 2D LC–MS via an online desalting step. We demonstrate the importance of such a setup for the determination of drug:antibody ratio (DAR), and the analysis of molecularity, fragmentation, and charge variants (deamidation, oxidation), notably under stress conditions. We discuss the advantages of 2D LC–MS in a regulated environment.

Latest:

A rapid, accurate, and precise method for the quantification of trypsin inhibitor activity was evaluated. The method utilizes alpha hydroxyl acid capped oligo-lysines [hydroxy acid (Lys)n] or alpha hydroxyl acid capped oligo-lysines-methionine [hydroxy acid (Lys-Met)] as substrates. Hydrolysis of the oligopeptides yields unique chemical residues that were readily quantified with electrospray–mass spectrometry (ESI-MS). Accuracy and precision of the approach compared favorably with that of the standard test method.

Latest:

Despite all of the recent advances in analytical technologies dedicated to biotherapeutics, accurate protein quantification remains a challenge for the biopharmaceutical industry. UV spectrophotometry is commonly used for batch testing, but it requires the knowledge of the extinction coefficient of the protein, whose experimental determination requires the accurate concentration of a reference standard obtained by an absolute quantification method. To address the need for a fast analytical method capable of accurately quantifying a protein without any specific reference substance, an isotope dilution ICP-MS method was developed and validated, based on sulfur determination, allowing very accurate determination of a single protein in solution after microwave digestion.

Latest:

Miniature X-ray sources reached the development level that is appropriate for their use in the handheld and portable X-Ray diffraction (XRD) instruments. This note describes the application of X-ray sources for the residual stress measurements using XRD.

Latest:

XRF is a key quality control technique for cement production. We look at results from two types of portland cement prepared as pressed pellets.

Latest:

This application note describes the measurement of food macronutrients by diamond attenuated total reflectance (ATR) mid infrared spectroscopy. Specific examples include the measurement of sugar content of grapes (grape juice) and the measurement of fats in cheese, chocolate and milk.

Latest:

This application note describes the measurement of food macronutrients by diamond attenuated total reflectance (ATR) mid infrared spectroscopy. Specific examples include the measurement of sugar content of grapes (grape juice) and the measurement of fats in cheese, chocolate and milk.

Latest:

Latest:

Monoclonal antibodies (mAbs) have been increasingly used as biotherapeutic agents and a number of new mAbs are currently in the drug pipeline. Over the next five years the patent on at least nine major biotherapeutic monoclonal antibodies will expire, opening the door for development and marketing of generic forms known as Biosimilars. In this paper a review of the central role mass spectrometry coupled to liquid chromatography plays in characterizing these antibodies is presented. Contemporary top down and middle-up approaches using mass spectrometry and various novel separation techniques to measure the intact masses of mAbs and their subunits or domains are highlighted. Example data of an innovator mAb, Humira (adalimumab) are presented showing the identities and relative abundances of the isoforms associated with this mAb. Similarly the current state of classical peptide mapping using reversed-phase chromatography and tandem mass spectrometry with scan- dependent acquisition is briefly reviewed. Novel approaches that speed analysis and provide information on post translational modifications, glycosylation, and disulfide mapping are discussed. Example data of stressed and unstressed samples of adalimumab are also presented to demonstrate peptide mapping data and modifications to the antibody. Lastly, the current use of mass spectrometry in glycoprofiling of mAbs is reviewed. Example glycan data for adalimumab generated by a novel labeling scheme and sensitive to detection by both fluorescence and mass spectrometry will be presented.

Latest:

Monoclonal antibodies (mAbs) have been increasingly used as biotherapeutic agents and a number of new mAbs are currently in the drug pipeline. Over the next five years the patent on at least nine major biotherapeutic monoclonal antibodies will expire, opening the door for development and marketing of generic forms known as Biosimilars. In this paper a review of the central role mass spectrometry coupled to liquid chromatography plays in characterizing these antibodies is presented. Contemporary top down and middle-up approaches using mass spectrometry and various novel separation techniques to measure the intact masses of mAbs and their subunits or domains are highlighted. Example data of an innovator mAb, Humira (adalimumab) are presented showing the identities and relative abundances of the isoforms associated with this mAb. Similarly the current state of classical peptide mapping using reversed-phase chromatography and tandem mass spectrometry with scan- dependent acquisition is briefly reviewed. Novel approaches that speed analysis and provide information on post translational modifications, glycosylation, and disulfide mapping are discussed. Example data of stressed and unstressed samples of adalimumab are also presented to demonstrate peptide mapping data and modifications to the antibody. Lastly, the current use of mass spectrometry in glycoprofiling of mAbs is reviewed. Example glycan data for adalimumab generated by a novel labeling scheme and sensitive to detection by both fluorescence and mass spectrometry will be presented.

Latest:

Monoclonal antibodies (mAbs) have been increasingly used as biotherapeutic agents and a number of new mAbs are currently in the drug pipeline. Over the next five years the patent on at least nine major biotherapeutic monoclonal antibodies will expire, opening the door for development and marketing of generic forms known as Biosimilars. In this paper a review of the central role mass spectrometry coupled to liquid chromatography plays in characterizing these antibodies is presented. Contemporary top down and middle-up approaches using mass spectrometry and various novel separation techniques to measure the intact masses of mAbs and their subunits or domains are highlighted. Example data of an innovator mAb, Humira (adalimumab) are presented showing the identities and relative abundances of the isoforms associated with this mAb. Similarly the current state of classical peptide mapping using reversed-phase chromatography and tandem mass spectrometry with scan- dependent acquisition is briefly reviewed. Novel approaches that speed analysis and provide information on post translational modifications, glycosylation, and disulfide mapping are discussed. Example data of stressed and unstressed samples of adalimumab are also presented to demonstrate peptide mapping data and modifications to the antibody. Lastly, the current use of mass spectrometry in glycoprofiling of mAbs is reviewed. Example glycan data for adalimumab generated by a novel labeling scheme and sensitive to detection by both fluorescence and mass spectrometry will be presented.

Latest:

Monoclonal antibodies (mAbs) have been increasingly used as biotherapeutic agents and a number of new mAbs are currently in the drug pipeline. Over the next five years the patent on at least nine major biotherapeutic monoclonal antibodies will expire, opening the door for development and marketing of generic forms known as Biosimilars. In this paper a review of the central role mass spectrometry coupled to liquid chromatography plays in characterizing these antibodies is presented. Contemporary top down and middle-up approaches using mass spectrometry and various novel separation techniques to measure the intact masses of mAbs and their subunits or domains are highlighted. Example data of an innovator mAb, Humira (adalimumab) are presented showing the identities and relative abundances of the isoforms associated with this mAb. Similarly the current state of classical peptide mapping using reversed-phase chromatography and tandem mass spectrometry with scan- dependent acquisition is briefly reviewed. Novel approaches that speed analysis and provide information on post translational modifications, glycosylation, and disulfide mapping are discussed. Example data of stressed and unstressed samples of adalimumab are also presented to demonstrate peptide mapping data and modifications to the antibody. Lastly, the current use of mass spectrometry in glycoprofiling of mAbs is reviewed. Example glycan data for adalimumab generated by a novel labeling scheme and sensitive to detection by both fluorescence and mass spectrometry will be presented.

Latest:

Latest:

Latest:

This application note describes the measurement of food macronutrients by diamond attenuated total reflectance (ATR) mid infrared spectroscopy. Specific examples include the measurement of sugar content of grapes (grape juice) and the measurement of fats in cheese, chocolate and milk.

Latest:

Fourier Transform Infrared (FTIR) microscopy is a well-established method for the analysis of samples that are too small or complex to be measured in a standard infrared spectrometer. The new LUMOS Stand-alone FTIR microscope offers a fully automated solution that is very easy-to-use and requires little space.

Latest:

In this study, a glycerol-fed, lab-scale E. coli bioprocess producing representative pharmaceutical compounds was monitored offline with a portable, high-sensitivity Raman spectrometer.

Latest:

Raman spectroscopy is particularly useful for identification of contaminant materials in pharmaceuticals because it can very clearly and nondestructively identify materials. Raman spectroscopy can be used to identify foreign matter on tablets as well as the individual tablet materials to confirm the material’s legitimacy. For injectable drug vials, Raman spectroscopy can be used with microscopy to count, size, and identify particulate contamination found in such vials. Spectral interpretation is key to the value of Raman spectroscopy, and it is important for accuracy of identification not to simply rely on library match values.

Latest:

The December meeting of the New York–New Jersey chapter of the Society for Applied Spectroscopy (NYSAS), held at the Metropolitan Museum of Art in New York (“The Met”), drew an audience of students and professionals to hear talks on material testing by Eric Breitung, PhD, a senior research scientist, and Catherine Stephens, PhD, an associate research scientist, both from The Met.

Latest:

Raman spectroscopy is particularly useful for identification of contaminant materials in pharmaceuticals because it can very clearly and nondestructively identify materials. Raman spectroscopy can be used to identify foreign matter on tablets as well as the individual tablet materials to confirm the material’s legitimacy. For injectable drug vials, Raman spectroscopy can be used with microscopy to count, size, and identify particulate contamination found in such vials. Spectral interpretation is key to the value of Raman spectroscopy, and it is important for accuracy of identification not to simply rely on library match values.

Latest:

Cosmetic preparations are common consumer products that consist of various organic and inorganic materials. In this paper, a method for the identification and spatial discrimination of the components in eye shadow samples using laser Raman microspectroscopy is described. The use of a multivariate curve resolution (MCR) is utilized during the analysis of the cosmetic preparation mapping data to develop the spatial discrimination information presented within this note.

Latest:

Aligned semiconducting single-walled carbon nanotubes (s-SWCNTs) are expected to outperform silicon as the next generation of integrated circuits. Greater utilization of polarized Raman spectroscopy is proving beneficial for efficient characterization of alignment in CNT films. Here, we present the results of how polarized Raman imaging can be used to effectively characterize alignment in large regions of aligned s-SWCNT films.

Latest:

Cosmetic preparations are common consumer products that consist of various organic and inorganic materials. In this paper, a method for the identification and spatial discrimination of the components in eye shadow samples using laser Raman microspectroscopy is described. The use of a multivariate curve resolution (MCR) is utilized during the analysis of the cosmetic preparation mapping data to develop the spatial discrimination information presented within this note.

Latest:

Aligned semiconducting single-walled carbon nanotubes (s-SWCNTs) are expected to outperform silicon as the next generation of integrated circuits. Greater utilization of polarized Raman spectroscopy is proving beneficial for efficient characterization of alignment in CNT films. Here, we present the results of how polarized Raman imaging can be used to effectively characterize alignment in large regions of aligned s-SWCNT films.

Latest:

Aligned semiconducting single-walled carbon nanotubes (s-SWCNTs) are expected to outperform silicon as the next generation of integrated circuits. Greater utilization of polarized Raman spectroscopy is proving beneficial for efficient characterization of alignment in CNT films. Here, we present the results of how polarized Raman imaging can be used to effectively characterize alignment in large regions of aligned s-SWCNT films.

Latest:

Cosmetic preparations are common consumer products that consist of various organic and inorganic materials. In this paper, a method for the identification and spatial discrimination of the components in eye shadow samples using laser Raman microspectroscopy is described. The use of a multivariate curve resolution (MCR) is utilized during the analysis of the cosmetic preparation mapping data to develop the spatial discrimination information presented within this note.