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Determination of Very Low Abundance Diagnostic Proteins in Serum Using Immunocapture LC–MS/MSThere is growing interest in the determination of endogenous proteins in biological samples for diagnostic purposes, because a concentration increase or decrease of such proteins can allows us to monitor the state of a pathological condition such as cancer. Immunocapture LC–MS/MS analysis combines the workflow of conventional immunological assays with LC–MS analysis. This article describes typical challenges, such as cross reactivity and the mass spectrometer’s dynamic range, as well as the advantages of isoform differentiation and multiplexing.
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Analysis of the Brill Transition and Reversible Brill Transition in Nylon 6,6 by Raman SpectroscopyThis article verified the Brill transition in nylon 6,6 by Raman spectroscopy through heating and cooling processes of the sample. When nylon is heated at around 160 C a crystalline phase transition occurs from a triclinic structure at room temperature to a pseudohexagonal structure above that temperature. This phase transition is known as the Brill Transition. With temperature-dependent Raman scattering measurements, it was possible to determine the vibrational behavior of nylon 6,6 during the Brill transition, and consequently to identify the main Raman bands associated with the Brill transition.
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Vibrational Spectroscopic Discrimination of Herbal Medicines: Polygala senega, Polygala tenuifolia, and Glinus oppositifoliusThe noninvasive discrimination of powdered root material belonging to the Polygala genus and an adulterant is presented. The quality of the approach is assessed for attenuated total reflectance mid-infrared spectroscopy and diffuse reflectance near-infrared spectroscopy. Due to the pharmaceutical importance of Polygala related plant material, conclusions are drawn towards a laboratory independent discrimination of the samples.
Latest:
Vibrational Spectroscopic Discrimination of Herbal Medicines: Polygala senega, Polygala tenuifolia, and Glinus oppositifoliusThe noninvasive discrimination of powdered root material belonging to the Polygala genus and an adulterant is presented. The quality of the approach is assessed for attenuated total reflectance mid-infrared spectroscopy and diffuse reflectance near-infrared spectroscopy. Due to the pharmaceutical importance of Polygala related plant material, conclusions are drawn towards a laboratory independent discrimination of the samples.
Latest:
Vibrational Spectroscopic Discrimination of Herbal Medicines: Polygala senega, Polygala tenuifolia, and Glinus oppositifoliusThe noninvasive discrimination of powdered root material belonging to the Polygala genus and an adulterant is presented. The quality of the approach is assessed for attenuated total reflectance mid-infrared spectroscopy and diffuse reflectance near-infrared spectroscopy. Due to the pharmaceutical importance of Polygala related plant material, conclusions are drawn towards a laboratory independent discrimination of the samples.
Latest:
Vibrational Spectroscopic Discrimination of Herbal Medicines: Polygala senega, Polygala tenuifolia, and Glinus oppositifoliusThe noninvasive discrimination of powdered root material belonging to the Polygala genus and an adulterant is presented. The quality of the approach is assessed for attenuated total reflectance mid-infrared spectroscopy and diffuse reflectance near-infrared spectroscopy. Due to the pharmaceutical importance of Polygala related plant material, conclusions are drawn towards a laboratory independent discrimination of the samples.
Latest:
Vibrational Spectroscopic Discrimination of Herbal Medicines: Polygala senega, Polygala tenuifolia, and Glinus oppositifoliusThe noninvasive discrimination of powdered root material belonging to the Polygala genus and an adulterant is presented. The quality of the approach is assessed for attenuated total reflectance mid-infrared spectroscopy and diffuse reflectance near-infrared spectroscopy. Due to the pharmaceutical importance of Polygala related plant material, conclusions are drawn towards a laboratory independent discrimination of the samples.
Latest:
Purity Analysis of Adulterated Essential Oils by FT-IR Spectroscopy and Partial-Least-Squares RegressionThis study reported a combined use of ordinary Fourier transform-infrared spectroscopy (FT-IR) in conjunction with partial-least-square (PLS) multivariate regression for accurate determination of the percent compositions of four essential oils (EOs) (wintergreen, tea tree, rosemary, and lemon eucalyptus oils) that were adulterated either with lemongrass essential oil (LO) or peppermint essential oil (PO). The FT-IR spectra of the calibration sample sets of known compositions of adulterated EOs with LO or PO were measured and subjected to PLS multivariate regression analysis. The simplicity, low-cost, and high accuracy of the protocol makes it appealing for routine industrial quality assurance of consumable goods.
Latest:
Purity Analysis of Adulterated Essential Oils by FT-IR Spectroscopy and Partial-Least-Squares RegressionThis study reported a combined use of ordinary Fourier transform-infrared spectroscopy (FT-IR) in conjunction with partial-least-square (PLS) multivariate regression for accurate determination of the percent compositions of four essential oils (EOs) (wintergreen, tea tree, rosemary, and lemon eucalyptus oils) that were adulterated either with lemongrass essential oil (LO) or peppermint essential oil (PO). The FT-IR spectra of the calibration sample sets of known compositions of adulterated EOs with LO or PO were measured and subjected to PLS multivariate regression analysis. The simplicity, low-cost, and high accuracy of the protocol makes it appealing for routine industrial quality assurance of consumable goods.
Latest:
Purity Analysis of Adulterated Essential Oils by FT-IR Spectroscopy and Partial-Least-Squares RegressionThis study reported a combined use of ordinary Fourier transform-infrared spectroscopy (FT-IR) in conjunction with partial-least-square (PLS) multivariate regression for accurate determination of the percent compositions of four essential oils (EOs) (wintergreen, tea tree, rosemary, and lemon eucalyptus oils) that were adulterated either with lemongrass essential oil (LO) or peppermint essential oil (PO). The FT-IR spectra of the calibration sample sets of known compositions of adulterated EOs with LO or PO were measured and subjected to PLS multivariate regression analysis. The simplicity, low-cost, and high accuracy of the protocol makes it appealing for routine industrial quality assurance of consumable goods.
Latest:
Purity Analysis of Adulterated Essential Oils by FT-IR Spectroscopy and Partial-Least-Squares RegressionThis study reported a combined use of ordinary Fourier transform-infrared spectroscopy (FT-IR) in conjunction with partial-least-square (PLS) multivariate regression for accurate determination of the percent compositions of four essential oils (EOs) (wintergreen, tea tree, rosemary, and lemon eucalyptus oils) that were adulterated either with lemongrass essential oil (LO) or peppermint essential oil (PO). The FT-IR spectra of the calibration sample sets of known compositions of adulterated EOs with LO or PO were measured and subjected to PLS multivariate regression analysis. The simplicity, low-cost, and high accuracy of the protocol makes it appealing for routine industrial quality assurance of consumable goods.
Latest:
Purity Analysis of Adulterated Essential Oils by FT-IR Spectroscopy and Partial-Least-Squares RegressionThis study reported a combined use of ordinary Fourier transform-infrared spectroscopy (FT-IR) in conjunction with partial-least-square (PLS) multivariate regression for accurate determination of the percent compositions of four essential oils (EOs) (wintergreen, tea tree, rosemary, and lemon eucalyptus oils) that were adulterated either with lemongrass essential oil (LO) or peppermint essential oil (PO). The FT-IR spectra of the calibration sample sets of known compositions of adulterated EOs with LO or PO were measured and subjected to PLS multivariate regression analysis. The simplicity, low-cost, and high accuracy of the protocol makes it appealing for routine industrial quality assurance of consumable goods.
Latest:
Tracking Microplastics in the Environment via FT-IR MicroscopyMicroplastics from clothing, abrasive action on plastics, or engineered microbeads as found in some exfoliating cosmetics are showing up in many environmental systems. FT-IR microscopy is a useful tool in the analysis of microplastics, providing visual information, particle counts, and particle identification.
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Sensitive, Rapid Estimation of Moxidectin in Cattle Hair by LC–MS-MSMoxidectin formulations help to reduce hair loss and irritation due to parasite worms in animals. So Estimation of Moxidectin in hair is important to evaluate therapeutic levels, distribution & accumulation, however estimation is also useful to evaluate harm to birds when they eat animal hair. Hence Moxidectin estimation is required for pharmacokinetic as well as environmental exposure study. Objective of the present work is to develop a rapid, selective method for the estimation of Moxidectin in Cattle Hair by LC-MS/MS. Oxcarbazepine used as a internal standard. Moxidectin extracted from cattle hair by liquid-liquid extraction using Sorenson’s Buffer as digestion solvent for incubation & methyl tert-butyl ether as an extraction solvent. Detection was performed over the range 0.026 to 1.000 ng/mG using MRM in positive polarity at unit resolution under turbo ion spray whereas separation was achieved on Kinetex 100 x 4.6 mm, 5u EVO C18 100A column with Methanol : 10mM Amonium formate pumped as gradient flow with 4.50min run time. Q1 is 640.45 whereas Q3 is sum of 528.50 and 498.50. Validation parameters shown reliable results. Method is applied for the estimation of Moxidectin in cattle Hair.
Latest:
Sensitive, Rapid Estimation of Moxidectin in Cattle Hair by LC–MS-MSMoxidectin formulations help to reduce hair loss and irritation due to parasite worms in animals. So Estimation of Moxidectin in hair is important to evaluate therapeutic levels, distribution & accumulation, however estimation is also useful to evaluate harm to birds when they eat animal hair. Hence Moxidectin estimation is required for pharmacokinetic as well as environmental exposure study. Objective of the present work is to develop a rapid, selective method for the estimation of Moxidectin in Cattle Hair by LC-MS/MS. Oxcarbazepine used as a internal standard. Moxidectin extracted from cattle hair by liquid-liquid extraction using Sorenson’s Buffer as digestion solvent for incubation & methyl tert-butyl ether as an extraction solvent. Detection was performed over the range 0.026 to 1.000 ng/mG using MRM in positive polarity at unit resolution under turbo ion spray whereas separation was achieved on Kinetex 100 x 4.6 mm, 5u EVO C18 100A column with Methanol : 10mM Amonium formate pumped as gradient flow with 4.50min run time. Q1 is 640.45 whereas Q3 is sum of 528.50 and 498.50. Validation parameters shown reliable results. Method is applied for the estimation of Moxidectin in cattle Hair.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
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Characterization and Determination of Irganox 1076 and 1010 in Polyethylene Using Thermal Desorption and Reactive Pyrolysis-GC-MSThis application note details a GC-MS-based analytical method for the qualitative and quantitative determination of Irganox 1076 and 1010 in polyethylene.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
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Visualize Your Process Streams in Real-Time with MS for Rapid Process DevelopmentThe Diablo 5000A RTGA-MS allows the process stream to be seen in real-time and provides quantitative data with reliable mass information. The result is visualization.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
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Creating a High-Throughput LC–MS-MS System Using Common ComponentsHow to create a liquid chromatography–tandem mass spectrometry (LC–MS-MS) system using mass spectrometers, a high performance liquid chromatography (HPLC) binary pump system, and an autosampler
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Applying LC with Low-Resolution MS/ MS and Subsequent Library Search for Reliable Compound Identification in Systematic Toxicological AnalysisSystematic toxicological analysis is an important step in medicolegal investigations of death, poisoning, and drug use. The primary goal is the detection and confirmation of potentially toxic compounds in evidence. This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.
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Applying LC with Low-Resolution MS/ MS and Subsequent Library Search for Reliable Compound Identification in Systematic Toxicological AnalysisSystematic toxicological analysis is an important step in medicolegal investigations of death, poisoning, and drug use. The primary goal is the detection and confirmation of potentially toxic compounds in evidence. This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.
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Shedding New Light on Forensic TimelinesThe development of novel, nondestructive technologies for rapid analysis of evidence discovered at crime scenes is pertinent to our criminal justice system to solve the millions of violent crimes that occur each year. Without a witness, establishing the precise timeline of a crime is difficult. Therefore, technologies based on sensitive, nondestructive techniques are needed to evaluate evidence and create such a timeline. We propose the use of Raman spectroscopy to analyze the age of bloodstains for potential use as a forensics tool. Previous studies have revealed Raman spectroscopy is sensitive to changes in blood analyte concentration and different oxidative states of hemoglobin. Raman spectroscopy could allow for rapid comparison of spectra from blood stains of unknown age to spectra of known age with high temporal accuracy. Though further investigation into other substrates and biochemical components should be performed, our study reveals Raman spectroscopy has the potential to accurately and nondestructively determine the age of a bloodstain for use in criminal investigations.
Latest:
Shedding New Light on Forensic TimelinesThe development of novel, nondestructive technologies for rapid analysis of evidence discovered at crime scenes is pertinent to our criminal justice system to solve the millions of violent crimes that occur each year. Without a witness, establishing the precise timeline of a crime is difficult. Therefore, technologies based on sensitive, nondestructive techniques are needed to evaluate evidence and create such a timeline. We propose the use of Raman spectroscopy to analyze the age of bloodstains for potential use as a forensics tool. Previous studies have revealed Raman spectroscopy is sensitive to changes in blood analyte concentration and different oxidative states of hemoglobin. Raman spectroscopy could allow for rapid comparison of spectra from blood stains of unknown age to spectra of known age with high temporal accuracy. Though further investigation into other substrates and biochemical components should be performed, our study reveals Raman spectroscopy has the potential to accurately and nondestructively determine the age of a bloodstain for use in criminal investigations.
Latest:
Shedding New Light on Forensic TimelinesThe development of novel, nondestructive technologies for rapid analysis of evidence discovered at crime scenes is pertinent to our criminal justice system to solve the millions of violent crimes that occur each year. Without a witness, establishing the precise timeline of a crime is difficult. Therefore, technologies based on sensitive, nondestructive techniques are needed to evaluate evidence and create such a timeline. We propose the use of Raman spectroscopy to analyze the age of bloodstains for potential use as a forensics tool. Previous studies have revealed Raman spectroscopy is sensitive to changes in blood analyte concentration and different oxidative states of hemoglobin. Raman spectroscopy could allow for rapid comparison of spectra from blood stains of unknown age to spectra of known age with high temporal accuracy. Though further investigation into other substrates and biochemical components should be performed, our study reveals Raman spectroscopy has the potential to accurately and nondestructively determine the age of a bloodstain for use in criminal investigations.
Latest:
Shedding New Light on Forensic TimelinesThe development of novel, nondestructive technologies for rapid analysis of evidence discovered at crime scenes is pertinent to our criminal justice system to solve the millions of violent crimes that occur each year. Without a witness, establishing the precise timeline of a crime is difficult. Therefore, technologies based on sensitive, nondestructive techniques are needed to evaluate evidence and create such a timeline. We propose the use of Raman spectroscopy to analyze the age of bloodstains for potential use as a forensics tool. Previous studies have revealed Raman spectroscopy is sensitive to changes in blood analyte concentration and different oxidative states of hemoglobin. Raman spectroscopy could allow for rapid comparison of spectra from blood stains of unknown age to spectra of known age with high temporal accuracy. Though further investigation into other substrates and biochemical components should be performed, our study reveals Raman spectroscopy has the potential to accurately and nondestructively determine the age of a bloodstain for use in criminal investigations.
Latest:
Shedding New Light on Forensic TimelinesThe development of novel, nondestructive technologies for rapid analysis of evidence discovered at crime scenes is pertinent to our criminal justice system to solve the millions of violent crimes that occur each year. Without a witness, establishing the precise timeline of a crime is difficult. Therefore, technologies based on sensitive, nondestructive techniques are needed to evaluate evidence and create such a timeline. We propose the use of Raman spectroscopy to analyze the age of bloodstains for potential use as a forensics tool. Previous studies have revealed Raman spectroscopy is sensitive to changes in blood analyte concentration and different oxidative states of hemoglobin. Raman spectroscopy could allow for rapid comparison of spectra from blood stains of unknown age to spectra of known age with high temporal accuracy. Though further investigation into other substrates and biochemical components should be performed, our study reveals Raman spectroscopy has the potential to accurately and nondestructively determine the age of a bloodstain for use in criminal investigations.
