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A breakthrough using a microfluidic interface to conduct sensitive time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis and study liquid surfaces in situ under vacuum conditions is described here.

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A critical review focused on the Raman spectroscopy of carbonaceous materials and of polymer-based nanocomposites that contain carbonaceous (nano) materials as fillers

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A fluoride-regeneration approach enables biomonitoring of chemical warfare nerve agents.

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When characterizing microscale objects using a Raman microscope, certain basic practical aspects of analytical instrument performance and experimental design should be taken into consideration.

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Raman spectroscopy is a rapidly expanding field, with modern Raman spectrometers offering labs higher ease of use and sensitivity. Furthermore, combining Raman spectroscopy with scanning electron microscopy (SEM) and fluorescence-lifetime imaging microscopy (FLIM) can enhance the technique for various applications.

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Strict and steady food control protects consumers against undesired contaminations and guarantees a high level of quality. This can be achieved by enforcing maximum allowable concentrations of hazardous substances. For simultaneous quantitative determination of the inorganic elements in wine, the ICP-MS technique is the preferred quality control tool. ICP-MS offers high sensitivity (trace detection), a wide dynamic range and a high sample throughput. In this study, commercially available red and white wines were investigated; 14 different elements were quantified simultaneously: arsenic, cadmium, caesium, copper, chromium, vanadium, iron, manganese, nickel, lead, selenium, tin, thallium and zinc. The developed ICP-MS method has a high accuracy, regardless of element concentration.

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In this study, recovery rates between 92% and 105%, combined with very low variations (RSD

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Strict and steady food control protects consumers against undesired contaminations and guarantees a high level of quality. This can be achieved by enforcing maximum allowable concentrations of hazardous substances. For simultaneous quantitative determination of the inorganic elements in wine, the ICP-MS technique is the preferred quality control tool. ICP-MS offers high sensitivity (trace detection), a wide dynamic range and a high sample throughput. In this study, commercially available red and white wines were investigated; 14 different elements were quantified simultaneously: arsenic, cadmium, caesium, copper, chromium, vanadium, iron, manganese, nickel, lead, selenium, tin, thallium and zinc. The developed ICP-MS method has a high accuracy, regardless of element concentration.

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Despite all of the recent advances in analytical technologies dedicated to biotherapeutics, accurate protein quantification remains a challenge for the biopharmaceutical industry. UV spectrophotometry is commonly used for batch testing, but it requires the knowledge of the extinction coefficient of the protein, whose experimental determination requires the accurate concentration of a reference standard obtained by an absolute quantification method. To address the need for a fast analytical method capable of accurately quantifying a protein without any specific reference substance, an isotope dilution ICP-MS method was developed and validated, based on sulfur determination, allowing very accurate determination of a single protein in solution after microwave digestion.

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Despite all of the recent advances in analytical technologies dedicated to biotherapeutics, accurate protein quantification remains a challenge for the biopharmaceutical industry. UV spectrophotometry is commonly used for batch testing, but it requires the knowledge of the extinction coefficient of the protein, whose experimental determination requires the accurate concentration of a reference standard obtained by an absolute quantification method. To address the need for a fast analytical method capable of accurately quantifying a protein without any specific reference substance, an isotope dilution ICP-MS method was developed and validated, based on sulfur determination, allowing very accurate determination of a single protein in solution after microwave digestion.

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Chronic kidney disease or kidney complication resulting from another systematic disorder can impact the organ’s blood filtering capability resulting in the passage of blood-born proteins through the kidneys and into urine.  Clinical analyses for blood proteins in urine are performed to assess proper kidney function or to monitor a diagnosed disorder.  Serum albumin is a common target in these clinical assays and detection of elevated SA levels in urine is termed Albuminuria. Because of normal variability in urine content and volume multiple measurements are often made in comparison to creatitine levels within the same urine sample and reported as a ratio (ACR).  Demonstrated here is a novel means for quantifying albumin and creatinine directly from the same urine sample using MALDI-TOF mass spectrometry.  Standard addition of albumin and deuterated creatinine (d3) into control urine produced a linear and quantitative response (R2 = 0.99 and 0.98) and is used to quantify both analytes across their clinically relevant ranges. This MS-based method represents a simple, fast, attractive alternative to currently clinical methods.

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Chronic kidney disease or kidney complication resulting from another systematic disorder can impact the organ’s blood filtering capability resulting in the passage of blood-born proteins through the kidneys and into urine.  Clinical analyses for blood proteins in urine are performed to assess proper kidney function or to monitor a diagnosed disorder.  Serum albumin is a common target in these clinical assays and detection of elevated SA levels in urine is termed Albuminuria. Because of normal variability in urine content and volume multiple measurements are often made in comparison to creatitine levels within the same urine sample and reported as a ratio (ACR).  Demonstrated here is a novel means for quantifying albumin and creatinine directly from the same urine sample using MALDI-TOF mass spectrometry.  Standard addition of albumin and deuterated creatinine (d3) into control urine produced a linear and quantitative response (R2 = 0.99 and 0.98) and is used to quantify both analytes across their clinically relevant ranges. This MS-based method represents a simple, fast, attractive alternative to currently clinical methods.

Latest:

Chronic kidney disease or kidney complication resulting from another systematic disorder can impact the organ’s blood filtering capability resulting in the passage of blood-born proteins through the kidneys and into urine.  Clinical analyses for blood proteins in urine are performed to assess proper kidney function or to monitor a diagnosed disorder.  Serum albumin is a common target in these clinical assays and detection of elevated SA levels in urine is termed Albuminuria. Because of normal variability in urine content and volume multiple measurements are often made in comparison to creatitine levels within the same urine sample and reported as a ratio (ACR).  Demonstrated here is a novel means for quantifying albumin and creatinine directly from the same urine sample using MALDI-TOF mass spectrometry.  Standard addition of albumin and deuterated creatinine (d3) into control urine produced a linear and quantitative response (R2 = 0.99 and 0.98) and is used to quantify both analytes across their clinically relevant ranges. This MS-based method represents a simple, fast, attractive alternative to currently clinical methods.

Latest:

Chronic kidney disease or kidney complication resulting from another systematic disorder can impact the organ’s blood filtering capability resulting in the passage of blood-born proteins through the kidneys and into urine.  Clinical analyses for blood proteins in urine are performed to assess proper kidney function or to monitor a diagnosed disorder.  Serum albumin is a common target in these clinical assays and detection of elevated SA levels in urine is termed Albuminuria. Because of normal variability in urine content and volume multiple measurements are often made in comparison to creatitine levels within the same urine sample and reported as a ratio (ACR).  Demonstrated here is a novel means for quantifying albumin and creatinine directly from the same urine sample using MALDI-TOF mass spectrometry.  Standard addition of albumin and deuterated creatinine (d3) into control urine produced a linear and quantitative response (R2 = 0.99 and 0.98) and is used to quantify both analytes across their clinically relevant ranges. This MS-based method represents a simple, fast, attractive alternative to currently clinical methods.

Latest:

Chronic kidney disease or kidney complication resulting from another systematic disorder can impact the organ’s blood filtering capability resulting in the passage of blood-born proteins through the kidneys and into urine.  Clinical analyses for blood proteins in urine are performed to assess proper kidney function or to monitor a diagnosed disorder.  Serum albumin is a common target in these clinical assays and detection of elevated SA levels in urine is termed Albuminuria. Because of normal variability in urine content and volume multiple measurements are often made in comparison to creatitine levels within the same urine sample and reported as a ratio (ACR).  Demonstrated here is a novel means for quantifying albumin and creatinine directly from the same urine sample using MALDI-TOF mass spectrometry.  Standard addition of albumin and deuterated creatinine (d3) into control urine produced a linear and quantitative response (R2 = 0.99 and 0.98) and is used to quantify both analytes across their clinically relevant ranges. This MS-based method represents a simple, fast, attractive alternative to currently clinical methods.

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This application note details a GC-MS-based analytical method for the qualitative and quantitative determination of Irganox 1076 and 1010 in polyethylene.

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Elena Hagemann, Product Manager for Process Spectroscopy at Metrohm USA, discusses a novel synchronized, automatic calibration data collector. This system eliminates the laborious calibration process of prediction model development without manual sampling. This capability allows moisture measurement systems to be calibrated at the factory down to approximately 7 ppm and to be installed in pipelines and reactors without additional calibration effort.

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A discussion of active pharmaceutical ingredient (API) selection, drug product development, and mass spectrometry instrumentation

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At SciX 2016 in Minneapolis, Minnesota, the Society for Applied Spectroscopy sponsored the special session “Analytical Chemists Easing World Poverty.” This session was founded in 2011 by SAS Past-President Diane Parry to highlight unmet measurement needs in developing nations. With the support of sponsors like the SAS, Spectroscopy magazine, and ChromAfrica, it has evolved into a popular session that examines a variety of topics ranging from technical solutions to instrumentation problems to cultural challenges of Westerners working in developing nations.

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Libraries of pre-computed ATR-FTIR mixture spectra and conventional ATR-FTIR polymer spectra were used to rapidly identify three major components from an ATR-FTIR spectrum of a sample labeled “EVA”.

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Portable instrumentation for Raman spectroscopy has rapidly evolved over the last decade, where sample testing that once occurred in the laboratory is now executed in the field (e.g. warehouse).   Portable Raman spectroscopy is a powerful technique for the rapid identification of diversely sourced raw materials used in pharmaceutical processing.  In addition to portability; reduced cost, rapid data acquisition and ease of use make this powerful technique attractive and accessible to both expert spectroscopists and non-specialists.  In most cases, the method development can be easily accomplished in the laboratory after which the instrument and methods are transferred to field for sample analysis or warehouse areas for inspection of incoming raw material.  Qualitative Raman methods for identification of raw materials typically utilize spectral libraries for sample to standard comparison.  When developing Raman spectral libraries for raw material identification, great care is required when considering critical factors (e.g. instrument type, Raman capability, container type, container interference, background interference, material variability) that can potentially influence the identity of the material.  This paper discusses portable Raman techniques and approaches for raw material identification, as well as key considerations for developing and validating Raman spectral libraries.

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Excitation-emission spectroscopy provides information of multiple sites in luminescent materials. As a wavelength selective technique, however, it may lead to prolonged acquisition times when high resolution is required. In this application note we demonstrate how this can be done faster, whilst maintaining high resolution.

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Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.

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Most plants used in traditional Chinese medicine must be processed before their medicinal usage; hence the effective ingredients may differ from those in the freshly harvested plant extracts. In this work, we present a fast and generic approach using sub-2-?m liquid chromatography–time-of-flight–mass spectrometry (sub-2-?m-LC–TOF-MS) coupled with multivariate statistical data analysis to systematically profile ingredient changes between fresh and processed samples of huang jing.

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Measuring silver (Ag) in seawater is challenging. A sensitive analytical procedure, using a simple automated flow injection system online coupled with ICP-MS, which is easy to be installed in an ordinary ICP-MS lab, is reported in this paper. Parameters including flow rate and duration, and the effects of the pH and dissolved organic matter (DOM) concentration and salinity were investigated. The standard addition method was used for the quantification. The linear range of the method was up to 1000 ng kg-1. For samples with various salinities the RSDs were

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Measuring silver (Ag) in seawater is challenging. A sensitive analytical procedure, using a simple automated flow injection system online coupled with ICP-MS, which is easy to be installed in an ordinary ICP-MS lab, is reported in this paper. Parameters including flow rate and duration, and the effects of the pH and dissolved organic matter (DOM) concentration and salinity were investigated. The standard addition method was used for the quantification. The linear range of the method was up to 1000 ng kg-1. For samples with various salinities the RSDs were

Latest:

Portable instrumentation for Raman spectroscopy has rapidly evolved over the last decade, where sample testing that once occurred in the laboratory is now executed in the field (e.g. warehouse).   Portable Raman spectroscopy is a powerful technique for the rapid identification of diversely sourced raw materials used in pharmaceutical processing.  In addition to portability; reduced cost, rapid data acquisition and ease of use make this powerful technique attractive and accessible to both expert spectroscopists and non-specialists.  In most cases, the method development can be easily accomplished in the laboratory after which the instrument and methods are transferred to field for sample analysis or warehouse areas for inspection of incoming raw material.  Qualitative Raman methods for identification of raw materials typically utilize spectral libraries for sample to standard comparison.  When developing Raman spectral libraries for raw material identification, great care is required when considering critical factors (e.g. instrument type, Raman capability, container type, container interference, background interference, material variability) that can potentially influence the identity of the material.  This paper discusses portable Raman techniques and approaches for raw material identification, as well as key considerations for developing and validating Raman spectral libraries.