Authors


Ian Hayward

Latest:

Using Raman spectroscopy to analyse Li-ion battery anodes

It is vital to be able to rapidly and easily analyse the composition and structure of anodes. The inVia is ideal for locating, discriminating, and quantifying the different forms of carbon present in anodes, even those with subtle variations in structure.


John F. Casey

Latest:

New Approaches in Sample Preparation and Precise Multielement Analysis of Crude Oils and Refined Petroleum Products Using Single-Reaction-Chamber Microwave Digestion and Triple-Quadrupole ICP-MS

October’s AP column highlights a team of geochemists at the University of Houston who have been developing methods to streamline multi-element analysis for a more complete fingerprinting of oils by using one sample preparation method utilizing a single reaction chamber microwave digestion system and then analyzing these solutions for major, and minor elements by ICP-OES and low abundance trace elements by triple quadrupole (QQQ) ICP-MS. Results to date using this approach have shown that complete elemental recovery and removal of organic matrices can be achieved safely and that up to 57 elements can be determined in oils with good accuracy and precision. Removal of organic matrices during digestion not only helps to limit the formation of polyatomic spectral interferences, but improves instrument stability and reduces carbon build in the sample introduction and interface regions, which have traditionally plagued “dilute and shoot” methods.


Yongjun Gao

Latest:

New Approaches in Sample Preparation and Precise Multielement Analysis of Crude Oils and Refined Petroleum Products Using Single-Reaction-Chamber Microwave Digestion and Triple-Quadrupole ICP-MS

October’s AP column highlights a team of geochemists at the University of Houston who have been developing methods to streamline multi-element analysis for a more complete fingerprinting of oils by using one sample preparation method utilizing a single reaction chamber microwave digestion system and then analyzing these solutions for major, and minor elements by ICP-OES and low abundance trace elements by triple quadrupole (QQQ) ICP-MS. Results to date using this approach have shown that complete elemental recovery and removal of organic matrices can be achieved safely and that up to 57 elements can be determined in oils with good accuracy and precision. Removal of organic matrices during digestion not only helps to limit the formation of polyatomic spectral interferences, but improves instrument stability and reduces carbon build in the sample introduction and interface regions, which have traditionally plagued “dilute and shoot” methods.


Jeremy Peters

Latest:

Protein Secondary Structure Determination Using Drop Coat Deposition Confocal Raman Spectroscopy

The accurate determination of protein structure is integral to the medical and pharmaceutical communities’ ability to understand disease, and develop drugs. Current techniques (CD, IR, Raman) for protein structure prediction provide results that can be poorly resolved, while high resolution techniques (NMR, X-ray crystallography) can be both costly and time-consuming. This work proposes the use of drop coat deposition confocal Raman spectroscopy (DCDCR), coupled with peak fitting of the Amide I spectral region (1620–1720 cm-1) for the accurate determination of protein secondary structure. Studies conducted on BSA and ovalbumin show that the predictions of secondary structure content within 1% of representative crystal structure data is possible for model proteins. The results clearly demonstrate that DCDCR has the potential to be effectively used to obtain accurate secondary structure distributions for proteins.


Eugene Park

Latest:

Protein Secondary Structure Determination Using Drop Coat Deposition Confocal Raman Spectroscopy

The accurate determination of protein structure is integral to the medical and pharmaceutical communities’ ability to understand disease, and develop drugs. Current techniques (CD, IR, Raman) for protein structure prediction provide results that can be poorly resolved, while high resolution techniques (NMR, X-ray crystallography) can be both costly and time-consuming. This work proposes the use of drop coat deposition confocal Raman spectroscopy (DCDCR), coupled with peak fitting of the Amide I spectral region (1620–1720 cm-1) for the accurate determination of protein secondary structure. Studies conducted on BSA and ovalbumin show that the predictions of secondary structure content within 1% of representative crystal structure data is possible for model proteins. The results clearly demonstrate that DCDCR has the potential to be effectively used to obtain accurate secondary structure distributions for proteins.


Ravi Kalyanaraman

Latest:

Protein Secondary Structure Determination Using Drop Coat Deposition Confocal Raman Spectroscopy

The accurate determination of protein structure is integral to the medical and pharmaceutical communities’ ability to understand disease, and develop drugs. Current techniques (CD, IR, Raman) for protein structure prediction provide results that can be poorly resolved, while high resolution techniques (NMR, X-ray crystallography) can be both costly and time-consuming. This work proposes the use of drop coat deposition confocal Raman spectroscopy (DCDCR), coupled with peak fitting of the Amide I spectral region (1620–1720 cm-1) for the accurate determination of protein secondary structure. Studies conducted on BSA and ovalbumin show that the predictions of secondary structure content within 1% of representative crystal structure data is possible for model proteins. The results clearly demonstrate that DCDCR has the potential to be effectively used to obtain accurate secondary structure distributions for proteins.


Anna Luczak

Latest:

Protein Secondary Structure Determination Using Drop Coat Deposition Confocal Raman Spectroscopy

The accurate determination of protein structure is integral to the medical and pharmaceutical communities’ ability to understand disease, and develop drugs. Current techniques (CD, IR, Raman) for protein structure prediction provide results that can be poorly resolved, while high resolution techniques (NMR, X-ray crystallography) can be both costly and time-consuming. This work proposes the use of drop coat deposition confocal Raman spectroscopy (DCDCR), coupled with peak fitting of the Amide I spectral region (1620–1720 cm-1) for the accurate determination of protein secondary structure. Studies conducted on BSA and ovalbumin show that the predictions of secondary structure content within 1% of representative crystal structure data is possible for model proteins. The results clearly demonstrate that DCDCR has the potential to be effectively used to obtain accurate secondary structure distributions for proteins.


Varsha Ganesh

Latest:

Protein Secondary Structure Determination Using Drop Coat Deposition Confocal Raman Spectroscopy

The accurate determination of protein structure is integral to the medical and pharmaceutical communities’ ability to understand disease, and develop drugs. Current techniques (CD, IR, Raman) for protein structure prediction provide results that can be poorly resolved, while high resolution techniques (NMR, X-ray crystallography) can be both costly and time-consuming. This work proposes the use of drop coat deposition confocal Raman spectroscopy (DCDCR), coupled with peak fitting of the Amide I spectral region (1620–1720 cm-1) for the accurate determination of protein secondary structure. Studies conducted on BSA and ovalbumin show that the predictions of secondary structure content within 1% of representative crystal structure data is possible for model proteins. The results clearly demonstrate that DCDCR has the potential to be effectively used to obtain accurate secondary structure distributions for proteins.


Zhen Long Zhang

Latest:

The pH Dependence of the SERS Spectra of Methyl Yellow in Silver Colloid

Surface plasmon resonance, charge-transfer resonance, and their combination determine the enhancement of surface-enhanced Raman scattering signals, and the varying intensities of the signal at different pH levels may result from the change in contributions of the combined system.


HORIBA Application Laboratory Team

Latest:

Ellipsometric Characterization and Modeling of Different Types of Nanoparticles

UVISEL ellipsometers have been used for the characterization of several systems of nanoparticles. This ellipsometric characterization involves the development of specific modeling tools available within DeltaPsi2 software.


Tim Michecl

Latest:

Consumer Products: Sunscreens, Moisturizers, Deodorants, and Antiperspirants (AP)

Milestone’s UltraWAVE SRC benchtop microwave digestion enables cosmetic chemists to digest up to 15 different sample types simultaneously at temperatures as high as 300°C, greatly simplifying product development workflow with an easy efficient digestion sequence.


Jan Knoop

Latest:

Determination of Contaminants in Wine Using an ICP-MS Technique

Strict and steady food control protects consumers against undesired contaminations and guarantees a high level of quality. This can be achieved by enforcing maximum allowable concentrations of hazardous substances. For simultaneous quantitative determination of the inorganic elements in wine, the ICP-MS technique is the preferred quality control tool. ICP-MS offers high sensitivity (trace detection), a wide dynamic range and a high sample throughput. In this study, commercially available red and white wines were investigated; 14 different elements were quantified simultaneously: arsenic, cadmium, caesium, copper, chromium, vanadium, iron, manganese, nickel, lead, selenium, tin, thallium and zinc. The developed ICP-MS method has a high accuracy, regardless of element concentration.


John McQuatters

Latest:

Mercury Determination in Tuna, CRM 463 by USDA Method CLG-MERC1.01 Using the QuickTrace® M-7600 CVAAS Mercury Analyzer

Teledyne Leeman Labs QuickTrace® M7600 was used to analyze and determine total elemental mercury (Hg0) concentration in Tuna (CRM 463) following the guidance in USDA Method CLG-MERC1.01 and the operating conditions.


Ashu Bansal

Latest:

Photoluminescence and Electroluminescence of Organic Optoelectronics

Photoluminescence and electroluminescence spectroscopy is a useful technique in the investigation of organic optoelectronic devices. The instrumentation for steady-state and time-resolved photoluminescence, as well as electroluminescence, is specified in this application note on organic solar cells.


A. Walch

Latest:

Matrix-Assisted Laser Desorption-Ionization Imaging Mass Spectrometry for Direct Tissue Analysis

A summary of the most recent advances in sample preparation, instrumentation, and data-processing techniques for MALDI-IMS


Weihang Yang

Latest:

New Approaches in Sample Preparation and Precise Multielement Analysis of Crude Oils and Refined Petroleum Products Using Single-Reaction-Chamber Microwave Digestion and Triple-Quadrupole ICP-MS

October’s AP column highlights a team of geochemists at the University of Houston who have been developing methods to streamline multi-element analysis for a more complete fingerprinting of oils by using one sample preparation method utilizing a single reaction chamber microwave digestion system and then analyzing these solutions for major, and minor elements by ICP-OES and low abundance trace elements by triple quadrupole (QQQ) ICP-MS. Results to date using this approach have shown that complete elemental recovery and removal of organic matrices can be achieved safely and that up to 57 elements can be determined in oils with good accuracy and precision. Removal of organic matrices during digestion not only helps to limit the formation of polyatomic spectral interferences, but improves instrument stability and reduces carbon build in the sample introduction and interface regions, which have traditionally plagued “dilute and shoot” methods.








Xiao-Ying Yu

Latest:

Probing Aqueous Surfaces by TOF-SIMS

A breakthrough using a microfluidic interface to conduct sensitive time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis and study liquid surfaces in situ under vacuum conditions is described here.


Alin Cristian Chipara

Latest:

Raman Spectroscopy of Carbonaceous Materials: A Concise Review

A critical review focused on the Raman spectroscopy of carbonaceous materials and of polymer-based nanocomposites that contain carbonaceous (nano) materials as fillers


Sandra A. Thomson

Latest:

Monitoring of Biological Matrices by GC–MS-MS for Chemical Warfare Nerve Agent Detection

A fluoride-regeneration approach enables biomonitoring of chemical warfare nerve agents.


Alexander Rzhevskii

Latest:

Basic Aspects of Experimental Design in Raman Microscopy

When characterizing microscale objects using a Raman microscope, certain basic practical aspects of analytical instrument performance and experimental design should be taken into consideration.


Renishaw

Latest:

The Latest Advances in Correlative Raman Imaging

Raman spectroscopy is a rapidly expanding field, with modern Raman spectrometers offering labs higher ease of use and sensitivity. Furthermore, combining Raman spectroscopy with scanning electron microscopy (SEM) and fluorescence-lifetime imaging microscopy (FLIM) can enhance the technique for various applications.


Ludivine Fromentoux

Latest:

Determination of Contaminants in Wine Using an ICP-MS Technique

Strict and steady food control protects consumers against undesired contaminations and guarantees a high level of quality. This can be achieved by enforcing maximum allowable concentrations of hazardous substances. For simultaneous quantitative determination of the inorganic elements in wine, the ICP-MS technique is the preferred quality control tool. ICP-MS offers high sensitivity (trace detection), a wide dynamic range and a high sample throughput. In this study, commercially available red and white wines were investigated; 14 different elements were quantified simultaneously: arsenic, cadmium, caesium, copper, chromium, vanadium, iron, manganese, nickel, lead, selenium, tin, thallium and zinc. The developed ICP-MS method has a high accuracy, regardless of element concentration.


Reynhardt Klopper

Latest:

Microwave Digestion for Elemental Impurities Analysis According to ICH and USP Guidelines

In this study, recovery rates between 92% and 105%, combined with very low variations (RSD


Johan Leinders

Latest:

Determination of Contaminants in Wine Using an ICP-MS Technique

Strict and steady food control protects consumers against undesired contaminations and guarantees a high level of quality. This can be achieved by enforcing maximum allowable concentrations of hazardous substances. For simultaneous quantitative determination of the inorganic elements in wine, the ICP-MS technique is the preferred quality control tool. ICP-MS offers high sensitivity (trace detection), a wide dynamic range and a high sample throughput. In this study, commercially available red and white wines were investigated; 14 different elements were quantified simultaneously: arsenic, cadmium, caesium, copper, chromium, vanadium, iron, manganese, nickel, lead, selenium, tin, thallium and zinc. The developed ICP-MS method has a high accuracy, regardless of element concentration.