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Latest:
Raman for Bioprocess MonitoringExplore the use of Raman to monitor a simple bioprocess, fermentation of glucose with yeast, qualitatively and quantitatively.
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A Priori Performance Estimation of Spatial Filtering in Raman Backscattering ExperimentsA straightforward numerical approach to estimate the performance of a spatial filter in Raman backscattering spectroscopy has been developed. This approach enabled the authors to determine an optimal hole diameter that balances spatial resolution and signal intensity.
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A Priori Performance Estimation of Spatial Filtering in Raman Backscattering ExperimentsA straightforward numerical approach to estimate the performance of a spatial filter in Raman backscattering spectroscopy has been developed. This approach enabled the authors to determine an optimal hole diameter that balances spatial resolution and signal intensity.
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Whisky Analysis with Raman Spectroscopy in the Near-Infrared Spectral Range: Comparison of 785– and 1064–nm ExcitationWe compare the advantages and disadvantages of each laser wavelength.
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Liquid Chromatography–Time-of-Flight Mass Spectrometry for Cannabinoid Profiling and Quantitation in Hemp Oil ExtractsThe method presented here allows for the accurate, precise, and robust speciation, profiling, and quantification of cannabinoids in hemp oil extracts and commercial cannabinoid products for research and development laboratories.
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Testing the Limits of Ion Mobility Mass Spectrometry to Compare a Nonbiological Complex Drug Product and Purported Generics—A Case Study with CopaxoneIon mobility mass spectrometry (IMMS) is a two-dimensional technique that allows separation of ionized molecules based on molecular size, shape, and mass‑to‑charge ratio (m/z). It has rapidly become a valuable application for analyzing isomeric compounds in a complex matrix (e.g., proteomic and lipidomic samples) or complex mixtures of structurally related and isobaric analytes (e.g., oil samples or polymer blends). IMMS was investigated as a possible technique to compare purported generic products with Copaxone®, a drug for treating relapsing‑remitting multiple sclerosis, which contains a very complex mixture of synthetic peptides. The analysis was performed on 15 randomly chosen batches of Copaxone® and 5 batches of purported generics that are marketed drugs in their country of origin. All samples were compared to a reference batch of Copaxone® (P53961) using Waters HDMS Compare software. The analysis produced heat maps that highlighted significant intensity differences in peptides at various m/z and drift times. A quantitative assessment of these heat maps was also performed by summing all the pixel values to produce a total pixel value (TPV). While the average TPV for the Copaxone® batches was 510811, the TPVs of the purported generics were 8-13 fold higher (2301682 to 4276572).
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Specular Reflection Spectroscopy with MicroviewingPrinted ink on a coated aluminum sample was analyzed using a specular reflection accessory with the unique capability of simultaneous sample viewing.
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Investigating Coatings Using Glow Discharge Spectrometry (Mar 2023)This application note shows how LECO’s GDS instruments are ideal for investigating PVD coatings and the factors that determine their protection of substrate.
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High-Throughput Structure-Based Profiling and Annotation of FlavonoidsA novel mass spectrometry-based flavonoid profiling workflow is applied to characterize and structurally annotate a large number of unknown flavonoids in fruit juice and vegetable juice samples.
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High-Throughput Quantitative Analysis of Vitamin D Using a Multiple Parallel LC–MS System Combined with Integrated On-Line SPEA newly developed high-throughput method for the quantitation of vitamin D using both multiplexed LC and on-line SPE is discussed.
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Identifying Textiles with Extended-Range Near Infrared SpectroscopyA compact, MEMS-based, single-photodetector NIR device, the NanoQuest, is used to distinguish different types of textiles.
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Trace-Level Detection of Explosives Using Sputtered SERS SubstratesThis study explores the use of a novel SERS substrate that can enhance the Raman signals of explosives that are present in picogram quantities in neat solutions using a visible laser wavelength and a compact Raman instrument.
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Monitoring Polymer Phase Transitions by Combining Rheology and Raman SpectroscopyThe in situ combination of rheometry and Raman spectroscopy allows for real-time, synchronized measurement of both physical and chemical material properties.
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Gaining Insight into Cocoa Butter Polymorph Formation Through In Situ Rheology and Raman SpectroscopyThe hyphenation of rheology and Raman spectroscopy enabled a more holistic depiction of the crystallization process and provided unique insights into the formation of cocoa butter polymorph form IV.
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Establishing a Calibration Procedure for the Energy-Shift Axis in Diverse Raman SpectrometersA procedure was developed to calibrate the wavenumber (energy shift) axis in Raman spectrometers, and it was tested in both portable and laboratory-based instruments.
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Establishing a Calibration Procedure for the Energy-Shift Axis in Diverse Raman SpectrometersA procedure was developed to calibrate the wavenumber (energy shift) axis in Raman spectrometers, and it was tested in both portable and laboratory-based instruments.
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Establishing a Calibration Procedure for the Energy-Shift Axis in Diverse Raman SpectrometersA procedure was developed to calibrate the wavenumber (energy shift) axis in Raman spectrometers, and it was tested in both portable and laboratory-based instruments.
Latest:
Establishing a Calibration Procedure for the Energy-Shift Axis in Diverse Raman SpectrometersA procedure was developed to calibrate the wavenumber (energy shift) axis in Raman spectrometers, and it was tested in both portable and laboratory-based instruments.
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See-Through Measurements of Illicit Substances in Commercial Containers with the TacticID®-1064 STThe TacticID-1064 ST has dedicated software and hardware designed to measure materials through both transparent and opaque containers. These through-barrier measurements remove the need for active sampling of potentially dangerous compounds such as fentanyl, leading to safer operations and reduced wait time for clear results. The 1064 nm laser is also an advantage for analyzing fluorescent or impure material. A Raman system with a 785 or 830 nm laser may generate fluorescence from these samples, which can overwhelm the Raman signal and make identification impossible. In this application note, we explore some of the capabilities of the TacticID-1064 ST.
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Real-Time Chemometric Analysis of Multicomponent Bioprocesses Using Raman SpectroscopyIn this study, a glycerol-fed, lab-scale E. coli bioprocess producing representative pharmaceutical compounds was monitored offline with a portable, high-sensitivity Raman spectrometer.
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1064-nm Raman: The Right Choice for Biological Samples?Interference from background fluorescence is a common challenge in Raman analysis. A study of three different types of biological samples was made to compare the ability of 785-nm and 1064-nm excitation to deal with this problem.
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1064-nm Raman: The Right Choice for Biological Samples?Interference from background fluorescence is a common challenge in Raman analysis. A study of three different types of biological samples was made to compare the ability of 785-nm and 1064-nm excitation to deal with this problem.
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Rapid Enantiodifferentation of Chiral Organophosphorus Compounds by 31P NMR Spectroscopy in the Presence of α-Cyclodextrin as the Chiral Solvating AgentThis method, for the evaluation of the enantiomeric purity of particular phosphonate derivatives, offers advantages in terms of cost, simplicity, and measurement speed.
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Rapid Enantiodifferentation of Chiral Organophosphorus Compounds by 31P NMR Spectroscopy in the Presence of α-Cyclodextrin as the Chiral Solvating AgentThis method, for the evaluation of the enantiomeric purity of particular phosphonate derivatives, offers advantages in terms of cost, simplicity, and measurement speed.
Latest:
Rapid Enantiodifferentation of Chiral Organophosphorus Compounds by 31P NMR Spectroscopy in the Presence of α-Cyclodextrin as the Chiral Solvating AgentThis method, for the evaluation of the enantiomeric purity of particular phosphonate derivatives, offers advantages in terms of cost, simplicity, and measurement speed.
Latest:
Rapid Enantiodifferentation of Chiral Organophosphorus Compounds by 31P NMR Spectroscopy in the Presence of α-Cyclodextrin as the Chiral Solvating AgentThis method, for the evaluation of the enantiomeric purity of particular phosphonate derivatives, offers advantages in terms of cost, simplicity, and measurement speed.
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Overcoming Struggles in Implementing Handheld Raman Spectroscopy Across the Manufacturing LineIndustry leaders answer this question: In what area will spectroscopy see the biggest growth in the next five years?
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Peptide Mapping of Monoclonal Antibodies and Antibody–Drug Conjugates Using Micro-Pillar Array Columns Combined with Mass SpectrometryThe structural complexity of monoclonal antibodies (mAbs) challenges the capabilities of even the most advanced chromatography and mass spectrometry techniques. This study examines the use of micro-pillar array columns in combination with mass spectrometry for peptide mapping of both mAbs and antibody–drug conjugates (ADCs).
