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Challenges in the Interpretation of Data from Hyphenated Techniques, Part I: General Principles (PDF)This first installment in a series about challenges and pitfalls in interpreting data from hyphenated techniques explores general concepts in initial approaches.
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Toward Quantitative Imaging Mass SpectrometryThe main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
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XPS Surface Characterization of Disposable Laboratory Gloves and the Transfer of Glove Components to Other SurfacesX-ray photoelectron spectroscopy (XPS) can be used to determine the contaminants present on laboratory gloves and to evaluate the type and amount of contamination transfer from gloves to other surfaces.
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Analysis of Terpenes in Cannabis Using Headspace Solid-Phase Microextraction and GC–MSHeadspace SPME combined with GC–MS for the qualitative and quantitative analysis of terpenes in cannabis offers several advantages compared to other methods. It does not require the use of organic solvents, does not coextract matrix, and provides additional means of peak identification and purity using spectral data. It is also a nondestructive method.
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Toward Quantitative Imaging Mass SpectrometryThe main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
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Non-Destructive Red Wine Measurement with Dispersive 1064 nm Raman SpectroscopyOwing to technological improvements spurred on by the telecommunications boom of the last decade, Raman spectroscopy has become much more accessible to users in all application areas, including agricultural, forensic, pharmaceutical, biomedical, and others.
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Gas Chromatography–Mass Spectrometry Characterization of Vegetable Oil–Derived Potent Antimicrobial AgentsUnder a suitable thermal oxidation regime, vegetable oils yield a mixture of volatile and semivolatile organics that exhibit very high antimicrobial activities against a variety of microbial species. Volatile and semivolatile products were characterized with GC–MS using electron ionization and chemical ionization. The thermal oxidation of vegetable oils resulted in the formation of an array of shortand medium-chain acids, aldehydes, and ketones that act synergistically to yield a potent antimicrobial disinfectant.
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EDXRF Analysis of Coal by FP MethodThe analysis of coal is demonstrated, with specific emphasis on the measurement of Na2O, using a Cartesian geometry energy dispersive X-ray fluorescence (EDXRF) spectrometer employing the Fundamental Parameters (FP) approach.
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Observing the Solar Spectrum at House of ScienceAnalysis of the temperature of the photosphere and determine the temperature by students using CCD spectrometers.
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Toward Quantitative Imaging Mass SpectrometryThe main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
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A Novel Approach to Measure Crop Plant Protein ExpressionCrop development to improve yield or disease resistance has been explored for centuries and the technologies to measure these improvements have subsequently become complex. The use of transgenes in crop plants is a more technically advanced approach than traditional breeding and the success of this approach is best assessed using modern techniques that accurately quantify the desired traits. Here, we applied targeted liquid chromatography–mass spectrometry (LC–MS) using synthetic stable isotope–labeled peptides to identify and quantify the relative levels of transgenic to native protein.
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A Diamond ATR-FTIR Study of NitrilesThis applications note compares the sensitivity of a single-reflection ATR to multiple-reflection ATR for the nitrile functional group infrared transition.
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Polar Vapor-Enhanced Separations with Planar Differential Mobility Spectrometry-Mass SpectrometryA brief historical overview of DMS, followed by a synopsis of the instrumentation, physics, and chemistry behind the separation principles
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Gas Chromatography–Mass Spectrometry Characterization of Vegetable Oil–Derived Potent Antimicrobial AgentsUnder a suitable thermal oxidation regime, vegetable oils yield a mixture of volatile and semivolatile organics that exhibit very high antimicrobial activities against a variety of microbial species. Volatile and semivolatile products were characterized with GC–MS using electron ionization and chemical ionization. The thermal oxidation of vegetable oils resulted in the formation of an array of shortand medium-chain acids, aldehydes, and ketones that act synergistically to yield a potent antimicrobial disinfectant.
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The Hyphenation of HILIC, Reversed-Phase HPLC, and Atmospheric-Pressure-Ionization MSHydrophilic interaction liquid chromatography (HILIC) and reversed-phase high performance liquid chromatography (HPLC) are complementary techniques in the separation of organic molecules with a broad band of polarity. Both separation variants can be operated easily with volatile and water-miscible solvents (for example, ammonium acetate aqueous solution and acetonitrile). Thus, a hyphenation of both liquid chromatographic techniques with atmospheric pressure ionization (API) and mass spectrometry (MS) seems to be a consequent step.
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Identification of Novel Psychoactive Substances Using Hyphenated Mass Spectrometric TechniquesGas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) techniques offer advantages in separating and confirming the identity of constituents in novel psychoactive substances.
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High Voltage Accuracy and Precision on Small Compact X-ray SourceMoxi™ X-ray source is calibrated to within ±250 V over all settings between 5 to 50 kV. This impressive accuracy and precision is achieved spectroscopically, by measuring an X-ray spectrum's bremsstrahlung edge and storing correction high voltage offset information on each source.
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Label-Free Chemical Detection in Micro-Fabricated Devices Using FT-IR Spectroscopic ImagingFourier transform infrared (FT-IR) spectroscopic imaging is a highly versatile technique that can be applied to a wide range of systems. This article summarizes some of the recent efforts developing applications of FT-IR imaging for microfluidics. The main advantage of FT-IR imaging compared to traditional imaging methods is that it is a label-free imaging technique.
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Accurate Mass: Why It's the Best Solution for Metabolite Identification in Discovery, Development, and Clinical ApplicationsAccurate-mass approaches offer a significant advance over nominal-mass approaches in the arena of qualitative analysis, and some of the analytical approaches can now be conducted in a relatively routine manner.
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Spectrophotometric Determination of Potassium Bromate Concentration in Bread via Redox Reaction With Promethazine in Acidic ConditionsPotassium bromate as an oxidizing agent in bread production is a staple, but it also poses health risks due to its classification as a carcinogen, necessitating careful monitoring of its residual levels. This application note reviews the spectrophotometric determination of potassium bromate concentration in bread based on the redox reaction between potassium bromate and promethazine in an acidic environment.
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Rapid Quantification of Trypsin Inhibitors in Food and Feed Formulations with Electrospray Ionization Mass SpectrometryA rapid, accurate, and precise method for the quantification of trypsin inhibitor activity was evaluated. The method utilizes alpha hydroxyl acid capped oligo-lysines [hydroxy acid (Lys)n] or alpha hydroxyl acid capped oligo-lysines-methionine [hydroxy acid (Lys-Met)] as substrates. Hydrolysis of the oligopeptides yields unique chemical residues that were readily quantified with electrospray–mass spectrometry (ESI-MS). Accuracy and precision of the approach compared favorably with that of the standard test method.
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The Application of Single-Reaction-Chamber Microwave Digestion to the Preparation of Pharmaceutical Samples in Accordance with USP <232> and <233>Single-reaction-chamber (SRC) microwave digestion was used for the digestion of pharmaceutical samples before inductively coupled plasma–mass spectrometry (ICP-MS) analysis according to draft USP chapters <232> and <233>.
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Analysis of Trace Elements as Impurities in Materials Used for Lithium-Ion Battery ProductionLithium-based batteries are key for moving away from the combustion of fossil fuels at the point of use. ICP-OES and ICP-MS methods can measure trace-element impurities that may affect battery performance.
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A Novel Approach to Measure Crop Plant Protein ExpressionCrop development to improve yield or disease resistance has been explored for centuries and the technologies to measure these improvements have subsequently become complex. The use of transgenes in crop plants is a more technically advanced approach than traditional breeding and the success of this approach is best assessed using modern techniques that accurately quantify the desired traits. Here, we applied targeted liquid chromatography–mass spectrometry (LC–MS) using synthetic stable isotope–labeled peptides to identify and quantify the relative levels of transgenic to native protein.
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Nontargeted, Discovery-Based Profiling of the Yeast Metabolome Using QTOF LC–MS and LC–MS-MSA discovery-based, untargeted metabolomics analysis of hundreds of yeast metabolites under robust, controlled extraction conditions followed by identification is described.
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A Novel Approach to Measure Crop Plant Protein ExpressionCrop development to improve yield or disease resistance has been explored for centuries and the technologies to measure these improvements have subsequently become complex. The use of transgenes in crop plants is a more technically advanced approach than traditional breeding and the success of this approach is best assessed using modern techniques that accurately quantify the desired traits. Here, we applied targeted liquid chromatography–mass spectrometry (LC–MS) using synthetic stable isotope–labeled peptides to identify and quantify the relative levels of transgenic to native protein.
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Advanced Analysis of Allergens in Foods: LC–MS-MS Methodologies and Future InsightsAn update on the sample preparation and LC–MS-MS tools available for allergen detection, as well advantages of those techniques.
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Identification of Organic Additives in Nitrile Rubber Materials by Pyrolysis-GC–MSNitrile rubber materials were studied using flash analytical pyrolysis-GC–MS to demonstrate that this technique is a good tool to identify the additives in nitrile rubber.
