This article provides a review of using both time- and phase-resolved fluorescence for DNA analysis in both sequencing and microarray applications.
In this short overview, we consider cases for diffuse reflection spectroscopy and introduce the Kubelka-Munk diffuse reflectance formula. We conclude by comparing diffuse transmittance, diffuse reflectance, logarithmic transforms of both, and the Kubelka-Munk transform for mid-infrared spectroscopy of the same sample.
An important attribute of a novel ionization process for use in mass spectrometry (MS) is its simplicity and flexibility to be hyphenated to conventional liquid-based separation methods.
SERS of bacterial cells can be a useful technique for clinical diagnostics, as illustrated by the analysis of a human urine sample spiked with urinary tract infection bacteria.
Whether the goal is food safety, ensuring quality, or establishing provenance, measuring the trace element content of foods and beverages that we all consume is of paramount importance. While some elements are essential for our well being at low concentrations, others like lead and chromium are highly toxic and more still are being linked to viral, neurological, and other diseases. Food scares related to contamination or poor quality not only constitute a health risk, they also undermine consumer confidence. This can lead to lost earnings through reduced sales and loss of credibility through adverse publicity.
One of the Cannabis Science Conference (CSC) Fall speakers is Autumn Phillips, who works as a Research Chemist and Chemical Hygiene Officer at Inorganic Ventures. She shares more about her background in cannabis science in this conference preview.
Infrared spectroscopy is an appealing technique for application to forensic samples because it offers the benefits of being non-destructive and non-hazardous, fast, reasonably sensitive, and resistant to some of the interferences of many commonly used techniques. Our research team has been focusing on detecting biological fluids on fabrics, which are inherently anisotropic substrates for spectroscopy. The work presented here investigates the effect of azimuthal angle of the sample on the infrared diffuse reflection spectra of fabrics with a goal of removing sampling differences as a source of analytic variation.
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Digestion of oils and plastics can be a challenge even to analytical laboratories. Herein, samples ranging from robust to routine are completely digested in minutes.
In this study, various samples of bread spreads including fruit spreads, peanut butter, nut butters, cocoa spreads, etc. were tested for heavy metal contamination.
The authors show that high resolution 1H nuclear magnetic resonance (NMR) spectroscopy can be used to study biofilm metabolism under environmentally relevant conditions in a minimally invasive way.
A brief historical overview of DMS, followed by a synopsis of the instrumentation, physics, and chemistry behind the separation principles
The main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
Cement is one of the most important materials in the construction industry. Traditionally, WDXRF spectrometers used in cement plants have been large, floor-standing models with substantial installation requirements and ownership expenses. This application note demonstrates the capabilities of the Rigaku Supermini - a new low-cost, benchtop wavelength dispersive X-ray fluorescence (WDXRF) spectrometer - for the rapid quantitative elemental analysis of cement raw meal.
The characterization of the active pharmaceutical ingredient (API) and its distribution and physical properties in commercial medicine is necessary in drug research and development process in the pharmaceutical industry. Among various analytical techniques employed for this purpose, Raman spectroscopy is gaining more popularity due to its advantages as non-destructive, non-invasive, fast spectrum acquisition in seconds, high reproducibility, and so on.
DIP–MS is a fast and easy tool that can identify classes of compounds in opportunity crudes (heavy and ultraheavy crude oils, asphaltenes, and tar sands) in the field, without prior separation or treatment. It may enable fast screening of real samples to make a rough evaluation of the potential of reservoirs and oil fields.
This work demonstrates the ability of the NexION® ICP-MS, coupled to the dedicated Syngistix™ Nano Application software module, to measure silver (Ag) nanoparticles in seawater and track their transformations over time.
The main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
Portable NIR spectroscopy is demonstrated as a rapid and mobile analysis method for authenticating cardiovascular medicines in critical situations, and to indicate whether formulations are counterfeit or substandard.
Raman and XRF spectroscopy were used to examine paint in artwork, revealing the potential of both techniques to verify the authenticity of famous works of art.
Owing to technological improvements spurred on by the telecommunications boom of the last decade, Raman spectroscopy has become much more accessible to users in all application areas, including agricultural, forensic, pharmaceutical, biomedical, and others.
Crop development to improve yield or disease resistance has been explored for centuries and the technologies to measure these improvements have subsequently become complex. The use of transgenes in crop plants is a more technically advanced approach than traditional breeding and the success of this approach is best assessed using modern techniques that accurately quantify the desired traits. Here, we applied targeted liquid chromatography–mass spectrometry (LC–MS) using synthetic stable isotope–labeled peptides to identify and quantify the relative levels of transgenic to native protein.
A brief historical overview of DMS, followed by a synopsis of the instrumentation, physics, and chemistry behind the separation principles
Crop development to improve yield or disease resistance has been explored for centuries and the technologies to measure these improvements have subsequently become complex. The use of transgenes in crop plants is a more technically advanced approach than traditional breeding and the success of this approach is best assessed using modern techniques that accurately quantify the desired traits. Here, we applied targeted liquid chromatography–mass spectrometry (LC–MS) using synthetic stable isotope–labeled peptides to identify and quantify the relative levels of transgenic to native protein.
A study of colony collapse disorder in honey bees illustrates how mass spectrometry–based proteomics techniques can be used to to identify pathogens without any prior knowledge of what is contained in the sample.
The main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
Clays, like kaolinite and smectite, are hygroscopic and it is well known that the adsorbed water can be driven off at elevated temperatures. At very low temperatures, in addition to the typical band narrowing and shifting, changes in the O-H bond vibrational modes of clays have also been observed.
Gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS) techniques offer advantages in separating and confirming the identity of constituents in novel psychoactive substances.