A more successful blueprint for analyzing Raman spectral data is outlined by following the 11 important steps, which are outlined here.
The “selective fluorescence quenching effects” of Fe3+ ions on carbon dots are examined to gain a more comprehensive understanding of the interactions of metal ions with a variety of fluorescent materials.
This study aimed to establish a fast, accurate method for quality evaluation of herbal medicine using NIR and chemometrics with ultraviolet-visible spectrophotometry (UV-vis) as a standard method to determine the total flavonoids content.
Regulations have been imposed to set legal limits of nitrate and nitrite in water worldwide. In this study, a highly accurate and optimized ultraviolet (UV) spectroscopy method is proposed to simultaneously monitor nitrate and nitrite for rapid determination and continuous monitoring in environmental water applications.
Raman measurements of chromite minerals demonstrated that chromium content could be accurately determined, supporting a possible application of portable Raman devices on Earth or in space for mineral analysis of asteroids and planets.
Photodynamic therapy is widely used as an established biomedical optical modality for the conservative treatment of tumors. This work investigates laser-induced fluorescence spectroscopy of the emerging photodynamic photosensitizer BODIPY-520 in turbid media.
Analysis of 66 pesticides and 5 mycotoxins regulated by the State of California in cannabis tinctures were analyzed using LC–MS/MS with an ESI source, and LC–MS/MS with an APCI source. A simple, fast, and cheap acetonitrile solvent extraction method was used for sample preparation for good recovery and high throughput, and internal standards were used to compensate for ion suppression effects from the hydrophobic matrix.
FT-IR offers an alternative method for the quantification and classification of lignocellulose in biofuel pellets, based on determination of cellulose, hemicellulose, and lignin content. The IR spectroscopic evaluation presented here provides an understanding of the pretreatment and storage of biofuel pellets.
We show how FT-IR may be used for quality control analysis of natrii sulfas, a transparent crystalline material used in natural medicine that primarily contains sodium sulfate decahydrate, crystallized from sulfate minerals.
Dmitry Kurouski, an associate professor of biomedical engineering in the department of Biochemistry and Biophysics at Texas A&M University in College Station, Texas, USA, recently spoke to Spectroscopy about Raman spectroscopy’s role in determining crop yield of key food items as the world population continues to increase.
We propose a theoretical basis using vis-NIR spectroscopy for the development of an online nondestructive testing system for the quality of Nanguo pear fruit.
The case studies presented here successfully demonstrate the use of inline Raman spectroscopic analysis to estimate solvent content during the solvent exchange and distillation operations in the synthesis of active pharmaceutical ingredients.
Metallomics seeks to understand the metallobiochemistry of cells and organisms in health and disease. This article explains the principle of laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) for imaging applications and highlights its potential to provide additional insights in bioanalysis and metallomics.
The use of high-resolution LIBS imaging requires the reduction of acquisition time. The authors describe a new developed system that accomplishes this goal and can be used in various applications where elemental composition and elemental distribution analysis is required.
The use of high-resolution LIBS imaging requires the reduction of acquisition time. The authors describe a new developed system that accomplishes this goal and can be used in various applications where elemental composition and elemental distribution analysis is required.
This study aimed to establish a fast, accurate method for quality evaluation of herbal medicine using NIR and chemometrics with ultraviolet-visible spectrophotometry (UV-vis) as a standard method to determine the total flavonoids content.
This study aimed to establish a fast, accurate method for quality evaluation of herbal medicine using NIR and chemometrics with ultraviolet-visible spectrophotometry (UV-vis) as a standard method to determine the total flavonoids content.
Researchers at Heriot-Watt University deploy the Chromacity OPO for active long-range FTIR spectroscopy to acquire high-resolution gas absorption spectra over 200 m.
Metallomics seeks to understand the metallobiochemistry of cells and organisms in health and disease. This article explains the principle of laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) for imaging applications and highlights its potential to provide additional insights in bioanalysis and metallomics.
In this paper, a one-time digestion method for the determination of arsenic (As), antimony (Sb), selenium (Se), and mercury (Hg) in geological samples was established.
In this short overview, we consider cases for diffuse reflection spectroscopy and introduce the Kubelka-Munk diffuse reflectance formula. We conclude by comparing diffuse transmittance, diffuse reflectance, logarithmic transforms of both, and the Kubelka-Munk transform for mid-infrared spectroscopy of the same sample.
A multiscale convolutional neural network (MsCNN) was used to screen Raman spectra of the hepatitis B serum, achieving higher classification accuracy compared to traditional machine learning methods.
This approach provides traceable and reliable quantitative elemental analysis of airborne particles for on-site environmental measurement with portable instrumentation.
We tackle one of the most common questions to arise when picking a Raman spectroscopy laser, “Should I choose a single, or multi-spatial mode laser for my application?”
Long chain fatty acids (LCFAs) function as a source of metabolic energy, substrates for membrane biogenesis, and storage of metabolic energy. Oxylipins, oxygenated derivatives of LCFAs, regulate the activity of many cellular processes. Existing methods for the analysis of LCFAs and oxylipins have limited compound coverage and sensitivity that, therefore, prevent their application in biological studies. In this work, we developed a high-throughput LC–MS method for analysis of 51 LCFAs and oxylipins. LCFAs and oxylipins were first extracted from biological samples via solid-phase extraction. The extracted molecules were analyzed by targeted comparative metabolomics. Saturated and monounsaturated LCFAs were analyzed in single ion reaction mode, while polyunsaturated LCFAs and oxylipins were analyzed in multiple reaction monitoring mode. Using this method, we successfully quantified 31 LCFAs and oxylipins from mouse livers.